A highly efficient method for concentrating DNA from river water by combined coagulation and foam separation

Both Escherichia coli and Enterococci were collected in foam within 7 min from 500 mL of bacteria-spiked water by coagulation and foam separation using ferric chloride and milk casein. These bacterial DNA isolated in the 100 µL of extract from the foam more than 87.5% recovery using the DNeasy Power...

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Bibliographic Details
Published in:Separation science and technology 2019-12, Vol.54 (18), p.3128-3134
Main Authors: Suzuki, Yoshihiro, Imafuku, Yuki, Nishiyama, Masateru, Teranishi, Kotaro, Jikumaru, Atsushi, Nukazawa, Kei, Ogura, Yoshitoshi
Format: Article
Language:English
Subjects:
16S rRNA gene sequencing analysis
Bacteria
Bacteria DNA
Casein
Coagulation
Deoxyribonucleic acid
DNA
DNA sequencing
E coli
Ferric chloride
Flora
foam concentration
Foam separation
Gene sequencing
Iron chlorides
Milk
Plants
River water
Rivers
rRNA 16S
Separation
Test procedures
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Summary:Both Escherichia coli and Enterococci were collected in foam within 7 min from 500 mL of bacteria-spiked water by coagulation and foam separation using ferric chloride and milk casein. These bacterial DNA isolated in the 100 µL of extract from the foam more than 87.5% recovery using the DNeasy PowerWater® Kit. To test this method with water from three natural rivers, 0.67-2.70 µg of DNA were concentrated in 100 µL of extract from 1,000 mL of river water. When the DNA extract was subjected to 16S rRNA gene sequencing analysis, information on the bacterial flora could be obtained.
ISSN:0149-6395
1520-5754
DOI:10.1080/01496395.2019.1565774