Cloning of cDNA encoding bovine follicle-stimulating hormone

The λgt10 library of bovine pituitary cDNA was screened and the clones with cDNA encoding α subunit of glycoprotein hormones were isolated. The sequencing of the inserts from two clones revealed that they containe 3'-terminal nucleotide of mRNA followed by the prolonged (about 300 bp) but diffe...

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Bibliographic Details
Published in:Biopolimery i kletka 1991-03, Vol.7 (2), p.80-86
Main Authors: Gorbulev, V. G., Chernov, B. K., Rubtsov, P. M.
Format: Article
Language:English
Subjects:
Cloning
Follicle-stimulating hormone
Menopause
mRNA
Pituitary
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Summary:The λgt10 library of bovine pituitary cDNA was screened and the clones with cDNA encoding α subunit of glycoprotein hormones were isolated. The sequencing of the inserts from two clones revealed that they containe 3'-terminal nucleotide of mRNA followed by the prolonged (about 300 bp) but different stretch. The clones carrying the cDNA for β subunit of follicle-stimulating hormone (FSH) were isolated from the enriched plasmid library of bovine pituitary cDNA. The sequence of cDNA for β subunit of FSH was established. The sequence results proved that we have cDNAs encoding the full-length both a and β subunits of bovine FSH.
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.0002C2