Individual culturing of preselected plant protoplasts and their fusion products in microdroplets

Successfull development of different plant species single cells, protoplasts and products of preselected single protoplast fusion has been shown using the method of individual culturing in microdroplets developed. The highest plating efficiency among the individual protoplasts reached about 75% (soy...

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Bibliographic Details
Published in:Biopolimery i kletka 1991-07, Vol.7 (4), p.6-20
Main Authors: Kirichenko, I. V., Gleba, Yu. Yu
Format: Article
Language:English
Subjects:
Plant cells
Protoplast fusion
Protoplasts
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Summary:Successfull development of different plant species single cells, protoplasts and products of preselected single protoplast fusion has been shown using the method of individual culturing in microdroplets developed. The highest plating efficiency among the individual protoplasts reached about 75% (soybean), the lowest one – 2,5% (clover). Electrofusion efficiency was as high as 95%. In this article we describe our individual culturing and electrofusion technology.
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.0002DA