Immunoelectron-microscopic study of seryl-tRNA-synthetase localization in cells of higher eucaryotes

Localization of seryl-tRNA-synthetase (SerRS) was studied on ultrathin (UT) section of bovine kidney cells fixed with glutaraldehyde and embedded in «Lowycril K4M» resin at – 35 °C. The UT sections were treated with the polyclonal affined-purified antibodies against SerRS and the complexes of protei...

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Bibliographic Details
Published in:Biopolimery i kletka 1990-09, Vol.6 (5), p.72-77
Main Authors: Sidorik, L. L., Popenko, V. I., Cherny, N. E., Tukalo, M. A., Beresten, S. F., Matsuka, G. Kh
Format: Article
Language:English
Subjects:
Cell size
Chromatin
Cytoplasm
Glutaraldehyde
Localization
Nuclei
tRNA
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Summary:Localization of seryl-tRNA-synthetase (SerRS) was studied on ultrathin (UT) section of bovine kidney cells fixed with glutaraldehyde and embedded in «Lowycril K4M» resin at – 35 °C. The UT sections were treated with the polyclonal affined-purified antibodies against SerRS and the complexes of protein A with colloidal gold 20 nm in size. In the cells the cytoplasm was the most intensely labelled. A great amount of SerRS was found in the cell nuclei: the label was concentrated over the diffuse chromatin localization regions, a minimal binding being observed over compact chromatin. The presence of SerRS in the eucaryotic cell nucleus may evidence for the possible regulatory role of SerRS in eucaryotes.
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.000290