A fast method for cloning of specific cDNA. Cloning of human prolactin cDNA

A method allowing fast and effective cloning of particular cDNA is described. Pairs of synthetic oligonucleotides-primers are used for polymerize chain reaction mediated selective amplification of prolactine cDNTA synthesized on human pituitary poly (A)-mRNA. Sequence of one of the recombinant plasm...

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Bibliographic Details
Published in:Biopolimery i kletka 1989-11, Vol.5 (6), p.87-92
Main Authors: Zolotukhin, S. B., Markelova, E. Yu, Panasenko, G. V., Naidenov, V. G., Shved, A. D., Sautin, Yu. Yu, Patsko, Ya. V.
Format: Article
Language:English
Subjects:
mRNA
Oligonucleotides
Pituitary
Prolactin
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Summary:A method allowing fast and effective cloning of particular cDNA is described. Pairs of synthetic oligonucleotides-primers are used for polymerize chain reaction mediated selective amplification of prolactine cDNTA synthesized on human pituitary poly (A)-mRNA. Sequence of one of the recombinant plasmid insert revealed a full-length pro-lactin cDNA, flanked by the synthetic primers.
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.0000FD