Effect of membrane-bound IgG and desialysation in the interaction of monocytes with senescent erythrocytes

Determination of the erythrocyte lifespan is a complex process affected by many cellular parameters. In the present study we measured and characterised the red blood cell (RBC) membrane proteins, mainly band 3, and quantified membrane-bound IgG in senescent RBC (SeRBC) and young RBC (YRBC). We also...

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Bibliographic Details
Published in:Clinical and experimental medicine 2006-10, Vol.6 (3), p.138-142
Main Authors: Ensinck, A, Biondi, C S, Marini, A, García Borrás, S, Racca, L L, Cotorruelo, C M, Racca, A L
Format: Article
Language:English
Subjects:
Biochemistry
Cellular biology
Diagnostic tests
Erythrocyte Aging - immunology
Erythrocyte Aging - physiology
Erythrocyte Membrane - immunology
Erythrocyte Membrane - metabolism
Humans
Immunoglobulin G - blood
In Vitro Techniques
Membranes
Monocytes - physiology
Phagocytosis
Proteins
Sialic Acids - blood
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Summary:Determination of the erythrocyte lifespan is a complex process affected by many cellular parameters. In the present study we measured and characterised the red blood cell (RBC) membrane proteins, mainly band 3, and quantified membrane-bound IgG in senescent RBC (SeRBC) and young RBC (YRBC). We also investigated, through a functional assay, the interaction between SeRBC and peripheral blood monocytes. We applied this erythrophagocytosis assay to study the phagocytosis of desialysed RBC. The results obtained showed no changes in the protein content between SeRBC and YRBC and no differences when examining membrane proteins by SDS-PAGE. Then, considering that the accumulation of autologous IgG on RBC membrane provides a direct mechanism for the removal of SeRBC, we measured the IgG content of intact RBC using an enzyme-linked anti-immunoglobulin test finding that the number of IgG molecules bound to SeRBC was significantly higher than that observed for YRBC. The increase observed in the percentage of erythrophagocytosis with SeRBC and sensitised RBC (SRBC) confirmed the involvement of autologous IgG in the selective removal of erythrocytes. We also observed a higher percentage of monocytes with phagocytosed and adherent RBC (AM) obtained with neuraminidase-treated RBC than those obtained with YRBC. This finding suggests that a decrease in sialic acid content of SeRBC may be involved in physiological erythrophagocytosis.
ISSN:1591-8890
1591-9528
DOI:10.1007/s10238-006-0110-y