The influence of pH alteration and pharmacological modulators of adenylate cyclasie system on human serum albumin conformation

Using the dynamic light scattering method It was found that albumin glohule has the most compact configuration (Stokes diameter 59–62 Å) at physiological pH 7.4. The changes in pH, both increase to 8.0 and decrease to 5.4, resulted in the growth of globule size to 72–81 Å. At acidic shift of pH an a...

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Bibliographic Details
Published in:Biopolimery i kletka 1999, Vol.15 (1), p.18-22
Main Authors: Luik, A. I., Naboka, Yu. N., Mogilevich, S. M., Hushcha, T. O., Mishchenko, N. I.
Format: Article
Language:English
Subjects:
Albumin
Carbachol
Clonidine
Conformation
Human serum albumin
Isoproterenol
Light scattering
pH effects
Propranolol
Theophylline
Yohimbine
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Summary:Using the dynamic light scattering method It was found that albumin glohule has the most compact configuration (Stokes diameter 59–62 Å) at physiological pH 7.4. The changes in pH, both increase to 8.0 and decrease to 5.4, resulted in the growth of globule size to 72–81 Å. At acidic shift of pH an additional peak arose in the correlation spectra caused by the light scattering on the structures with the Stokes diameters of 29–37 Å. Those conform to the sizes of the albumin subdomains. The indicated peak is not displayed at basic shift of pH. The interaction with propranolol, clonidine and carbachol, which hinder adenytate cyclase signaling system of a cell initiated structural rearrangements similar to acidic transitions. Isoproterenol, yohimbine and theophylline, which activate AdC, caused the conformaiional changes of HSA similar to basic transition.
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.000500