Cloning and overexpression of the part of envelope protein El gene of classical swine fever virus (Strain Shi-Min) in Escherichia coli

A fragment of El gene of virulent strain Shi-Min of classical swine fever virus(CSFV) 775 bp in length has been cloned using reverse transcriptase-polymerase chain reaction (RT-PCR). Primary sequence of the cloned fragment has been determined. This fragment has been fused to glutathione-S-transferas...

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Bibliographic Details
Published in:Biopolimery i kletka 1996, Vol.12 (5), p.93-99
Main Authors: Kirilenko, S. D., Deriabin, O. M., Kirilenko, O. L., Deriabina, E. G., Busol, V. O.
Format: Article
Language:English
Subjects:
Amino acid sequence
Cloning
Fever
Glutathione transferase
Hog cholera
Molecular weight
Polymerase chain reaction
Proteins
RNA-directed DNA polymerase
Viral envelope proteins
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Summary:A fragment of El gene of virulent strain Shi-Min of classical swine fever virus(CSFV) 775 bp in length has been cloned using reverse transcriptase-polymerase chain reaction (RT-PCR). Primary sequence of the cloned fragment has been determined. This fragment has been fused to glutathione-S-transferase and expressed in E. coli. Molecular weight of the part of El protein was estimated to be 31 kD as expected. The recombinant protein has been found mainly in insoluble fraction, its yield was up to 15% of the total cellular protein. Possible use of the protein is discussed.
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.00044F