Trp144 as a fluorescence probe for investigation of the C-module rapid conformation dynamics in eukaryotic tyrosyle-tRNA synthetase

The spectra of fluorescence of the tyrosyle-tRNA synthetase C-module have been obtained. The microsurrounding of a tryptophan residue has been characterized using the methods of fluorescence spectroscopy and computational modeling. The rapid conformation dynamics of the C-module in nanosecond time s...

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Bibliographic Details
Published in:Biopolimery i kletka 2003, Vol.19 (5), p.436-439
Main Authors: Kordysh, M. A., Odynets, K. A., Kornelyuk, A. I.
Format: Article
Language:English
Subjects:
Acrylamide
Computer applications
Conformation
Fluorescence spectroscopy
Spectrum analysis
tRNA
Tryptophan
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Summary:The spectra of fluorescence of the tyrosyle-tRNA synthetase C-module have been obtained. The microsurrounding of a tryptophan residue has been characterized using the methods of fluorescence spectroscopy and computational modeling. The rapid conformation dynamics of the C-module in nanosecond time scale is revealed using the fluorescence quenching with acrylamide and Cs ions.
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.000671