CoA Synthase influences adherence-independent growth and survival of mammalian cells in vitro

Aims. We aimed to study the influence of the expression level, activity and subcellular localization of CoA Synthase (CoASy) on anchoring independent growth and viability of in vitro cultured cells. Methods. Abilities of cells to form colonies in semisolid agarose and survive in growth factor deplet...

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Bibliographic Details
Published in:Biopolimery i kletka 2009, Vol.25 (5), p.384-389
Main Authors: Breus, O. S., Nemazanyy, I. O., Gout, I. T., Filonenko, V. V., Panasyuk, G. G.
Format: Article
Language:English
Subjects:
Cell lines
Localization
Mammalian cells
Mitochondria
Phenotypes
Signal transduction
siRNA
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Summary:Aims. We aimed to study the influence of the expression level, activity and subcellular localization of CoA Synthase (CoASy) on anchoring independent growth and viability of in vitro cultured cells. Methods. Abilities of cells to form colonies in semisolid agarose and survive in growth factor depleted conditions were tested. A panel of HEK293 stable cell lines which over express wild type, catalytically inactive or mitochondria association mutants of CoASy were used in the study. Effects of CoASy down regulation by siRNA on malignant phenotype of HepG2 cells have been studied. Results. We report here that changes in CoASy expression level affect anchoring independent growth and viability of mammalian cells. Catalytic activity of CoASy and its association with mitochondria are crucial for mediating of the observed effects. Conclusions. Presented data indicate CoASy has positive impact on activity of signalling pathways in the cell and reveal unknown before functional link between signal transduction and metabolism.
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.0007F0