Development of an vivo Himar1 transposon mutagenesis system for use in Streptococcus equi subsp. equi

Streptococcus equi subsp. equi is the causative agent of the equine disease strangles. In this study we describe the development of an in vivo Himar1 transposon system for the random mutagenesis of S. equi and, potentially, other Gram-positive bacteria. We demonstrate efficient and random transposit...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 2004-09, Vol.238 (2), p.401-409
Main Authors: May, J.P, Walker, C.A, Maskell, D.J, Slater, J.D
Format: Article
Language:English
Subjects:
animal pathogenic bacteria
Bacteria
Bacteriology
Biological and medical sciences
Chromosomes
DNA Transposable Elements - genetics
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Electroporation
Fundamental and applied biological sciences. Psychology
Genetic Vectors - chemistry
Genetic Vectors - genetics
Genetics
Gram-positive bacteria
Himar1
In vivo methods and tests
In vivo transposon mutagenesis
Microbiology
Mutagenesis
Mutagenesis, Insertional - methods
pathogenesis
Plasmids
Random mutagenesis
Strangles
Streptococcus
Streptococcus equi
Streptococcus equi - chemistry
Streptococcus equi - genetics
Streptococcus suis - chemistry
Streptococcus suis - genetics
Transposition
Transposon mutagenesis
Transposons
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Streptococcus equi subsp. equi is the causative agent of the equine disease strangles. In this study we describe the development of an in vivo Himar1 transposon system for the random mutagenesis of S. equi and, potentially, other Gram-positive bacteria. We demonstrate efficient and random transposition of a modified mini-transposon onto the chromosome by Southern blot analysis and insertion site sequencing. Non-haemolytic mutants were isolated at a frequency of 0.2%, and acapsular mutants at a frequency of 0.04%. Taken together, these data demonstrate that in vivo Himar1 mutagenesis can be used for genomic-scale mutational analysis of S. equi, and is likely to be applicable to the study of other streptococci.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2004.tb09782.x