The influence of alkylating agent MNNG on mutagenic effect of exogeneous recombinant DNA

The possible mutagenic activity of three recombinant plasmids pBR322ins, pAins, pGins has been investigated in this work. In three days after transfection two (pBR322ins, pAins) out of three recombinant plasmids revealed mutagenic effect in Chinese hamster cells. The mutation frequency decreased to...

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Bibliographic Details
Published in:Biopolimery i kletka 1995-01, Vol.11 (1), p.87-91
Main Authors: Lukash, L. L., Podolskaya, S. V., Suhorada, H. M., Kostetskaya, K. V., Kostetsky, I. E., Varzanova, I. S., Patskovsky, Yu. V., Vavilina, I. V., Deis, S. V.
Format: Article
Language:English
Subjects:
Genomes
Insulin
Pain
Plasmids
Recombinant DNA
Transfection
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Summary:The possible mutagenic activity of three recombinant plasmids pBR322ins, pAins, pGins has been investigated in this work. In three days after transfection two (pBR322ins, pAins) out of three recombinant plasmids revealed mutagenic effect in Chinese hamster cells. The mutation frequency decreased to ninth day after transfection. The mutagenic activity of recombinant plasmids presumably depends an the presence of nucleotide sequences which were coming from parental plasmids but not on insulin gene from human genome. Mutagenic activity of pAins probably was determined by the presence of Alu sequence that was recloned from plasmid pALl. The alkylating agent MNNG increased the mutagenic effect of exogeneaus DNA on 10th day after transfection.
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.0003D8