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The influence of alkylating agent MNNG on mutagenic effect of exogeneous recombinant DNA

The possible mutagenic activity of three recombinant plasmids pBR322ins, pAins, pGins has been investigated in this work. In three days after transfection two (pBR322ins, pAins) out of three recombinant plasmids revealed mutagenic effect in Chinese hamster cells. The mutation frequency decreased to...

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Published in:	Biopolimery i kletka 1995-01, Vol.11 (1), p.87-91
Main Authors:	Lukash, L. L., Podolskaya, S. V., Suhorada, H. M., Kostetskaya, K. V., Kostetsky, I. E., Varzanova, I. S., Patskovsky, Yu. V., Vavilina, I. V., Deis, S. V.
Format:	Article
Language:	English
Subjects:	Genomes Insulin Pain Plasmids Recombinant DNA Transfection
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creator	Lukash, L. L. Podolskaya, S. V. Suhorada, H. M. Kostetskaya, K. V. Kostetsky, I. E. Varzanova, I. S. Patskovsky, Yu. V. Vavilina, I. V. Deis, S. V.
description	The possible mutagenic activity of three recombinant plasmids pBR322ins, pAins, pGins has been investigated in this work. In three days after transfection two (pBR322ins, pAins) out of three recombinant plasmids revealed mutagenic effect in Chinese hamster cells. The mutation frequency decreased to ninth day after transfection. The mutagenic activity of recombinant plasmids presumably depends an the presence of nucleotide sequences which were coming from parental plasmids but not on insulin gene from human genome. Mutagenic activity of pAins probably was determined by the presence of Alu sequence that was recloned from plasmid pALl. The alkylating agent MNNG increased the mutagenic effect of exogeneaus DNA on 10th day after transfection.
doi_str_mv	10.7124/bc.0003D8
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issn	0233-7657 1993-6842
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source	Publicly Available Content (ProQuest)
subjects	Genomes Insulin Pain Plasmids Recombinant DNA Transfection
title	The influence of alkylating agent MNNG on mutagenic effect of exogeneous recombinant DNA
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