Characterization of extended-spectrum-β-lactamase (CTX-M-15)-producing strains of Salmonella enterica isolated in France and Senegal

• Published in: FEMS microbiology letters 2004-09, Vol.238 (2), p.353-358
• Main Authors: Weill, François-Xavier, Perrier-Gros-Claude, Jean-David, Demartin, Marie, Coignard, Sophie, Grimont, Patrick A.D.
• Format: Article
• Language: English
• Subjects: Aminoglycosides; Clinical isolates; CTX‐M‐15; E coli; Electrophoresis; ESBL; France; Gastroenteritis; Gel electrophoresis; Microbiology; Plasmids; Pulsed-field gel electrophoresis; Ribotyping; Salmonella; Salmonella enterica; Senegal; Sulfamethoxazole; Trimethoprim; Urinary tract; Urine
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.2004.tb09776.x

Abstract Three clinical isolates of Salmonella enterica recovered between 2000 and 2003 in France and Senegal were found to produce extended-spectrum β-lactamase CTX-M-15. The two isolates from Senegal were recovered from stool of a hospitalized patient with gastroenteritis in 2000 and from an urine specimen of an out-patient with urinary tract infection in 2001. These S. enterica isolates belonged to serotype Kentucky and were clonally related as determined by pulsed-field gel electrophoresis and automated ribotyping. The third isolate of serotype Typhimurium was recovered from a patient hospitalized in France for an acute gastroenteritis acquired in Lebanon. The blaCTX-M-15 gene was located on two different transferable plasmids, one of which also carried blaTEM-1, blaOXA-30, aminoglycoside-, tetracycline-, and sulfamethoxazole-trimethoprim resistance genes. ISEcp1 element was found to be located upstream of blaCTX-M-15 in the same position as reported previously in CTX-M-15-producing Escherichia coli from India and Turkey. This is the first report of blaCTX-M-15 in the genus Salmonella.