Study of tobacco mosaic virus by atomic force microscope

The paper presents the methodological approaches to the use of an atomic force microscope to study the viral nature of objects and their interaction with specific immunoglobulin (IgG). Application formvar coating mica chips enhances the adsorption of virions virtually affecting the surface topograph...

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Bibliographic Details
Published in:Biopolimery i kletka 1999-09, Vol.15 (5), p.456-460
Main Authors: Polischuk, V. P., Rengevych, O. V., Beketov, G. V., Tyvonchuk, T. P., Boyko, A. L.
Format: Article
Language:English
Subjects:
Atomic force microscopy
Immunoglobulin G
Microscopes
Virions
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Summary:The paper presents the methodological approaches to the use of an atomic force microscope to study the viral nature of objects and their interaction with specific immunoglobulin (IgG). Application formvar coating mica chips enhances the adsorption of virions virtually affecting the surface topography image of the phase shift STM interaction with IgG, which is proportional to Strength of adhesion of the probe to the surface, allowed visualization of the distribution of surface antibodies viral particles. The method is unique in terms of virology, as it removes the fixation preparation required for research in the transmission microscope
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.00053F