Preparation of the fluorescein reagent for solid-phase oligonucleotide 5'-labelling and its use for the synthesis of fluorescently labelled PCR primers for HIV-1 detection

The preparative synthesis of fluorescein H-phosphonate reagent for solid phase 'labelling of oligonucteotides is described. Fluorescein-labelted thymidine and two PCR primers for HIV-1 detection were synthesized using this reagent.

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Bibliographic Details
Published in:Biopolimery i kletka 1995-07, Vol.11 (3-4), p.35-41
Main Authors: Dubey, I. Ya, Fedoryak, O. D., Tkachak, O. M., Fedoryak, D. M.
Format: Article
Language:English
Subjects:
Fluorescein
HIV
Human immunodeficiency virus
Labeling
Oligonucleotides
Phosphonates
Thymidine
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Description
Summary:The preparative synthesis of fluorescein H-phosphonate reagent for solid phase 'labelling of oligonucteotides is described. Fluorescein-labelted thymidine and two PCR primers for HIV-1 detection were synthesized using this reagent.
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.0003EB