The introduction of the 9[alpha]-hydroxy group into androst-4-en-3,17-dione using a new actinobacterium strain

A 9α-hydrolase activity of a new actinobacterium strain identified as Rhodococcus erythropolis based on the analysis of a 16S rRNA gene sequence (1417 nucleotides) towards androst-4-en-3,17-dione (AD) was studied. In the presence of glucose in the medium, this strain completely transformed AD (4-20...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2009-07, Vol.45 (4), p.395
Main Authors: Rodina, N V, Andryushina, V A, Stytsenko, T S, Turova, T P, Baslerov, R V, Panteleeva, A N, Voishvillo, N E
Format: Article
Language:English
Subjects:
Bacteria
Biochemistry
Bioengineering
Enzymes
Online Access:Get full text
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Summary:A 9α-hydrolase activity of a new actinobacterium strain identified as Rhodococcus erythropolis based on the analysis of a 16S rRNA gene sequence (1417 nucleotides) towards androst-4-en-3,17-dione (AD) was studied. In the presence of glucose in the medium, this strain completely transformed AD (4-20 g/l) into 9α-hydroxy-AD over 20-48 h. This culture was able to grow and perform AD 9α-hydroxylation at a concentration of dimethyl formamide up to 9%. Crystalline 9α-hydroxy-AD was isolated with a yield of over 90%. [PUBLICATION ABSTRACT]
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683809040085