Possible quorum sensing in the rumen microbial community: detection of quorum-sensing signal molecules from rumen bacteria

The bioluminescence assay using Vibrio harveyi BB170 was used to examine quorum-sensing autoinducer 2 (AI-2) activity from cell-free culture fluids of rumen bacteria. The assay showed that the culture fluids of four species of rumen bacteria, Butyrivibrio fibrisolvens, Eubacterium ruminantium, Rumin...

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Bibliographic Details
Published in:FEMS microbiology letters 2003-02, Vol.219 (1), p.47-52
Main Authors: Mitsumori, Makoto, Xu, Liming, Kajikawa, Hiroshi, Kurihara, Mitsunori, Tajima, Kiyoshi, Jin, Hai, Takenaka, Akio
Format: Article
Language:English
Subjects:
Bacteria
Bioluminescence
Cell culture
Cell signaling
Detection
Fluids
Homology
LuxS protein
Microbiology
Microorganisms
Quorum sensing
Rumen
Waterborne diseases
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Summary:The bioluminescence assay using Vibrio harveyi BB170 was used to examine quorum-sensing autoinducer 2 (AI-2) activity from cell-free culture fluids of rumen bacteria. The assay showed that the culture fluids of four species of rumen bacteria, Butyrivibrio fibrisolvens, Eubacterium ruminantium, Ruminococcus flavefaciens, and Succinimonas amylolytica, contained AI-2-like molecules. Furthermore, homologues for luxS genes were detected in rumen fluids collected from three cows and in bacterial cells of P. ruminicola subsp. ruminicola and R. flavefaciens. These findings suggest that the quorum-sensing system mediated by AI-2 is present in the rumen.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-10970201192-8