Phylogenetic analysis and confirmation of the endospore-forming nature of Pasteuria penetrans based on the spo0A gene

Pasteuria penetrans is an obligate parasite of plant parasitic nematodes and has yet to be grown in vitro. We have cloned the pivotal sporulation gene, spo0A, which is the first whole gene yet to come from this organism. Partial spo0A sequences were also obtained from the related bacteria, Pasteuria...

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Bibliographic Details
Published in:FEMS microbiology letters 2003-08, Vol.225 (2), p.249-256
Main Authors: Trotter, James R, Bishop, Alistair H
Format: Article
Language:English
Subjects:
Biological control
Gene sequencing
Heterogeneity
Microbiology
Nematodes
Parasites
Phylogenetics
Phylogeny
Spo0A gene
Sporulation
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Summary:Pasteuria penetrans is an obligate parasite of plant parasitic nematodes and has yet to be grown in vitro. We have cloned the pivotal sporulation gene, spo0A, which is the first whole gene yet to come from this organism. Partial spo0A sequences were also obtained from the related bacteria, Pasteuria ramosa and Alicyclobacillus acidocaldarius. Phylogenetic analyses using the spo0A sequence data from this and previous studies confirmed the closeness of the genera Pasteuria and members of the supergenus Bacillus. A segment of the spo0A gene was also used to show that genetic heterogeneity exists within and between populations of P. penetrans. This may explain, partly at least, the variability of P. penetrans as a biological control agent of nematodes.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-10970300528-7