Virulence and genetic diversity among Fusarium oxysporum f. sp. carthami isolates of India using multilocus RAPD and ISSR markers

Safflower wilt, incited by Fusarium oxysporum f. sp . carthami (Foc) is one of the most devastating diseases of safflower. Virulence and genetic diversity of 90 Foc isolates representing four major safflower growing states (Maharashtra, Karnataka, Telangana, and Madhya Pradesh) in India were analyze...

Full description

Saved in:
Bibliographic Details
Published in:Tropical plant pathology 2019-10, Vol.44 (5), p.409-422
Main Authors: Singh, Neeraja, Anand, Garima, Kapoor, Rupam
Format: Article
Language:English
Subjects:
Admixtures
Alleles
Biomedical and Life Sciences
Carthamus tinctorius
Clusters
Cultivars
Fusarium oxysporum
Genetic diversity
Heterozygosity
Life Sciences
Markers
Original Article
Plant Pathology
Population genetics
Population structure
Quadrants
Races
Random amplified polymorphic DNA
Virulence
Wilt
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Safflower wilt, incited by Fusarium oxysporum f. sp . carthami (Foc) is one of the most devastating diseases of safflower. Virulence and genetic diversity of 90 Foc isolates representing four major safflower growing states (Maharashtra, Karnataka, Telangana, and Madhya Pradesh) in India were analyzed. Out of 45 RAPD and 45 ISSR primers screened, 17 of each RAPD and ISSR markers revealed a comprehensive picture of genetic diversity. Primers depicting maximum heterozygosity, effective number of alleles, and Shannon information index were identified. Cluster and principal coordinate analyses distributed isolates from a given state among different clusters and quadrants, respectively, indicating high genetic diversity. However, certain region-specific groupings within clusters were found. The population structure comprised two sub-populations with admixture of alleles. AMOVA indicated maximum molecular variation within states (95%), and within races (79%). Among the six races identified on the basis of disease reactions on differential cultivars, Race 6 was the most virulent and Race 2 as the most prevalent. Groupings on the basis of molecular markers and geographical origin showed some degree of concurrence with the virulence of isolates. The findings are useful in screening of newly developed pathogen resistant varieties for the deployment of region-specific resistant cultivars of safflower.
ISSN:1983-2052
1982-5676
1983-2052
DOI:10.1007/s40858-019-00303-1