Gibberellin A₃ detection in plants with high-performance liquid chromatography

A new method of gibberellin A₃ detection based on high-performance liquid chromatography has been elaborated. It combines high accuracy and low cost, and includes the following stages: fixation with liquid nitrogen; extraction of alcohol-soluble substances; purification with freezing, reextraction i...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2009-07, Vol.45 (4), p.454-458
Main Authors: Nefed'eva, E. E, Mazey, N. G
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Chromatography
Flowers & plants
Freezing
Life Sciences
Liquid chromatography
Medical Microbiology
Microbiology
Plant biology
Thin layer chromatography
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Summary:A new method of gibberellin A₃ detection based on high-performance liquid chromatography has been elaborated. It combines high accuracy and low cost, and includes the following stages: fixation with liquid nitrogen; extraction of alcohol-soluble substances; purification with freezing, reextraction in ethyl acetate, and thin-layer chromatography; and detection with HPLC. Chromatographic conditions and methods of substance identification have been determined. The method allows the use of modest amounts of plant material (5-20 g), at which variability does not exceed 7%, and the total loss of substance is approximately 3%. The results of gibberellin A₃ detection in buckwheat are presented.
ISSN:0003-6838
1608-3024
DOI:10.1134/S000368380904019X