Modified xylE and xylTE reporter genes for use in Streptomyces: analysis of the effect of xylT

Abstract The reporter gene xylE (encoding catechol 2,3-dioxygenase) has been modified for a more rational use in Streptomyces. Two reporter fragments, one containing xylE, and the other containing also the upstream gene xylT (which encodes a soluble ferredoxin), have been constructed to allow precis...

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Bibliographic Details
Published in:FEMS microbiology letters 2001-03, Vol.196 (2), p.229-234
Main Authors: González-Cerón, Gabriela, Licona, Paula, Servín-González, Luis
Format: Article
Language:English
Subjects:
Catechol
Catechol 2,3-dioxygenase
Copy number
Ferredoxin
Fragments
Genes
Microbiology
mRNA
Plasmids
Regulatory sequences
Reporter gene
Streptomyces
Streptomyces reporter gene
xylE gene
xylT gene
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Summary:Abstract The reporter gene xylE (encoding catechol 2,3-dioxygenase) has been modified for a more rational use in Streptomyces. Two reporter fragments, one containing xylE, and the other containing also the upstream gene xylT (which encodes a soluble ferredoxin), have been constructed to allow precise fusion of regulatory regions to the reporter genes. Identical fusions of these xylE and xylTE reporter fragments to the Streptomyces dagA and tipA promoters, in low and high copy number plasmids, show that the levels of xylE mRNA and catechol 2,3-dioxygenase activities are significantly higher when xylT is present.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2001.tb10569.x