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Cloning and characterization of the gene encoding penicillin-binding protein A of Streptomyces griseus
Abstract An internal segment of the penicillin-binding protein gene, pbpA, of Streptomyces griseus was amplified from genomic DNA using the polymerase chain reaction and used as a hybridization probe to isolate the complete gene from a cosmid library. pbpA encodes a 485 amino acid sequence that cons...
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| Published in: | FEMS microbiology letters 2000-12, Vol.193 (1), p.63-68 |
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| container_end_page | 68 |
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| container_title | FEMS microbiology letters |
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| creator | Jiang, Hao Kendrick, Kathleen E. |
| description | Abstract
An internal segment of the penicillin-binding protein gene, pbpA, of Streptomyces griseus was amplified from genomic DNA using the polymerase chain reaction and used as a hybridization probe to isolate the complete gene from a cosmid library. pbpA encodes a 485 amino acid sequence that conserves three motifs of PBPs, SXXK, SXN, and KTG. The pbpA gene was located downstream of a gene homologous to the Bacillus subtilis spoVE gene. The pbpA gene was disrupted by replacing an ApaI fragment of the pbpA gene in S. griseus chromosome with an apramycin resistance gene cassette or directly inserting this apramycin resistance gene cassette at the NcoI site of pbpA penicillin-binding domain. No obvious defects in growth, sporulation, or spore sonication resistance were observed in the constructed pbpA mutants, suggesting that PBPA is not essential for growth and sporulation under normal laboratory conditions in S. griseus. |
| doi_str_mv | 10.1111/j.1574-6968.2000.tb09403.x |
| format | article |
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An internal segment of the penicillin-binding protein gene, pbpA, of Streptomyces griseus was amplified from genomic DNA using the polymerase chain reaction and used as a hybridization probe to isolate the complete gene from a cosmid library. pbpA encodes a 485 amino acid sequence that conserves three motifs of PBPs, SXXK, SXN, and KTG. The pbpA gene was located downstream of a gene homologous to the Bacillus subtilis spoVE gene. The pbpA gene was disrupted by replacing an ApaI fragment of the pbpA gene in S. griseus chromosome with an apramycin resistance gene cassette or directly inserting this apramycin resistance gene cassette at the NcoI site of pbpA penicillin-binding domain. No obvious defects in growth, sporulation, or spore sonication resistance were observed in the constructed pbpA mutants, suggesting that PBPA is not essential for growth and sporulation under normal laboratory conditions in S. griseus.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2000.tb09403.x</identifier><identifier>PMID: 11094280</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Motifs ; Amino Acid Sequence ; Amino acids ; Antibiotics ; Apramycin ; Bacterial Proteins ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Blotting, Southern ; Carrier Proteins - chemistry ; Carrier Proteins - genetics ; Carrier Proteins - physiology ; Chromosomes ; Cloning ; Cloning, Molecular ; Deoxyribonucleic acid ; DNA ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Genetics ; Hexosyltransferases ; Homology ; Hybridization ; Microbiology ; Molecular Sequence Data ; Muramoylpentapeptide Carboxypeptidase - chemistry ; Muramoylpentapeptide Carboxypeptidase - genetics ; Muramoylpentapeptide Carboxypeptidase - physiology ; Mutagenesis, Insertional ; PbpA gene ; Penicillin ; Penicillin-binding protein ; Penicillin-binding protein A ; Penicillin-Binding Proteins ; Peptidyl Transferases ; Phenotype ; Polymerase Chain Reaction ; Protein A ; Proteins ; Sonication ; Spores, Bacterial - physiology ; Sporulation ; Streptomyces griseus ; Streptomyces griseus - chemistry ; Streptomyces griseus - genetics ; Streptomyces griseus - physiology</subject><ispartof>FEMS microbiology letters, 2000-12, Vol.193 (1), p.63-68</ispartof><rights>2000 Federation of European Microbiological Societies 2000</rights><rights>2000 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-135d9805dd49d648268958c7934d7842ff85b57b9ac3516a151eaa9e3e9ac9913</citedby><cites>FETCH-LOGICAL-c464t-135d9805dd49d648268958c7934d7842ff85b57b9ac3516a151eaa9e3e9ac9913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5692014$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11094280$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Hao</creatorcontrib><creatorcontrib>Kendrick, Kathleen E.</creatorcontrib><title>Cloning and characterization of the gene encoding penicillin-binding protein A of Streptomyces griseus</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
An internal segment of the penicillin-binding protein gene, pbpA, of Streptomyces griseus was amplified from genomic DNA using the polymerase chain reaction and used as a hybridization probe to isolate the complete gene from a cosmid library. pbpA encodes a 485 amino acid sequence that conserves three motifs of PBPs, SXXK, SXN, and KTG. The pbpA gene was located downstream of a gene homologous to the Bacillus subtilis spoVE gene. The pbpA gene was disrupted by replacing an ApaI fragment of the pbpA gene in S. griseus chromosome with an apramycin resistance gene cassette or directly inserting this apramycin resistance gene cassette at the NcoI site of pbpA penicillin-binding domain. No obvious defects in growth, sporulation, or spore sonication resistance were observed in the constructed pbpA mutants, suggesting that PBPA is not essential for growth and sporulation under normal laboratory conditions in S. griseus.</description><subject>Amino Acid Motifs</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Antibiotics</subject><subject>Apramycin</subject><subject>Bacterial Proteins</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Carrier Proteins - chemistry</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - physiology</subject><subject>Chromosomes</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genetics</subject><subject>Hexosyltransferases</subject><subject>Homology</subject><subject>Hybridization</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Muramoylpentapeptide Carboxypeptidase - chemistry</subject><subject>Muramoylpentapeptide Carboxypeptidase - genetics</subject><subject>Muramoylpentapeptide Carboxypeptidase - physiology</subject><subject>Mutagenesis, Insertional</subject><subject>PbpA gene</subject><subject>Penicillin</subject><subject>Penicillin-binding protein</subject><subject>Penicillin-binding protein A</subject><subject>Penicillin-Binding Proteins</subject><subject>Peptidyl Transferases</subject><subject>Phenotype</subject><subject>Polymerase Chain Reaction</subject><subject>Protein A</subject><subject>Proteins</subject><subject>Sonication</subject><subject>Spores, Bacterial - physiology</subject><subject>Sporulation</subject><subject>Streptomyces griseus</subject><subject>Streptomyces griseus - chemistry</subject><subject>Streptomyces griseus - genetics</subject><subject>Streptomyces griseus - physiology</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNp9kU9v1DAQxS0EotuFr4Ai4Jpgx39ic6tWpSBV4lA4W44z2XqVtRfbkVo-PY42ai-AL5Zm3pt5_hmh9wQ3pJxPh4bwjtVCCdm0GOMm91gxTJuHF2jz1HqJNph2siZYdRfoMqVDkbIWi9fogpQiayXeoHE3Be_8vjJ-qOy9icZmiO63yS74KoxVvodqDx4q8DYMi_IE3lk3Tc7XvfPnUgwZnK-uFsddjnDK4fhoIVX76BLM6Q16NZopwdv13qKfX65_7L7Wt99vvu2ubmvLBMs1oXxQEvNhYGoQTLZCKi5tpygbOsnacZS8512vjKWcCEM4AWMUUCgVpQjdog_nuSXRrxlS1ocwR19W6paWVwtWZhXV57PKxpBShFGfojua-KgJ1gtifdALR71w1AtivSLWD8XM_2PGROi7hbteuOviLNAZLdu36N0abe6PMDy71r8ogo-rwCRrpjEab1160nGhWkzY8_4wn_6Ru_5b7j9dqqXn</recordid><startdate>20001201</startdate><enddate>20001201</enddate><creator>Jiang, Hao</creator><creator>Kendrick, Kathleen E.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>20001201</creationdate><title>Cloning and characterization of the gene encoding penicillin-binding protein A of Streptomyces griseus</title><author>Jiang, Hao ; Kendrick, Kathleen E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-135d9805dd49d648268958c7934d7842ff85b57b9ac3516a151eaa9e3e9ac9913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Motifs</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Antibiotics</topic><topic>Apramycin</topic><topic>Bacterial Proteins</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Carrier Proteins - chemistry</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - physiology</topic><topic>Chromosomes</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genetics</topic><topic>Hexosyltransferases</topic><topic>Homology</topic><topic>Hybridization</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Muramoylpentapeptide Carboxypeptidase - chemistry</topic><topic>Muramoylpentapeptide Carboxypeptidase - genetics</topic><topic>Muramoylpentapeptide Carboxypeptidase - physiology</topic><topic>Mutagenesis, Insertional</topic><topic>PbpA gene</topic><topic>Penicillin</topic><topic>Penicillin-binding protein</topic><topic>Penicillin-binding protein A</topic><topic>Penicillin-Binding Proteins</topic><topic>Peptidyl Transferases</topic><topic>Phenotype</topic><topic>Polymerase Chain Reaction</topic><topic>Protein A</topic><topic>Proteins</topic><topic>Sonication</topic><topic>Spores, Bacterial - physiology</topic><topic>Sporulation</topic><topic>Streptomyces griseus</topic><topic>Streptomyces griseus - chemistry</topic><topic>Streptomyces griseus - genetics</topic><topic>Streptomyces griseus - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Hao</creatorcontrib><creatorcontrib>Kendrick, Kathleen E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Hao</au><au>Kendrick, Kathleen E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and characterization of the gene encoding penicillin-binding protein A of Streptomyces griseus</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2000-12-01</date><risdate>2000</risdate><volume>193</volume><issue>1</issue><spage>63</spage><epage>68</epage><pages>63-68</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
An internal segment of the penicillin-binding protein gene, pbpA, of Streptomyces griseus was amplified from genomic DNA using the polymerase chain reaction and used as a hybridization probe to isolate the complete gene from a cosmid library. pbpA encodes a 485 amino acid sequence that conserves three motifs of PBPs, SXXK, SXN, and KTG. The pbpA gene was located downstream of a gene homologous to the Bacillus subtilis spoVE gene. The pbpA gene was disrupted by replacing an ApaI fragment of the pbpA gene in S. griseus chromosome with an apramycin resistance gene cassette or directly inserting this apramycin resistance gene cassette at the NcoI site of pbpA penicillin-binding domain. No obvious defects in growth, sporulation, or spore sonication resistance were observed in the constructed pbpA mutants, suggesting that PBPA is not essential for growth and sporulation under normal laboratory conditions in S. griseus.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>11094280</pmid><doi>10.1111/j.1574-6968.2000.tb09403.x</doi><tpages>6</tpages></addata></record> |
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| source | Oxford Journals Online |
| subjects | Amino Acid Motifs Amino Acid Sequence Amino acids Antibiotics Apramycin Bacterial Proteins Bacteriology Base Sequence Biological and medical sciences Blotting, Southern Carrier Proteins - chemistry Carrier Proteins - genetics Carrier Proteins - physiology Chromosomes Cloning Cloning, Molecular Deoxyribonucleic acid DNA Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetics Hexosyltransferases Homology Hybridization Microbiology Molecular Sequence Data Muramoylpentapeptide Carboxypeptidase - chemistry Muramoylpentapeptide Carboxypeptidase - genetics Muramoylpentapeptide Carboxypeptidase - physiology Mutagenesis, Insertional PbpA gene Penicillin Penicillin-binding protein Penicillin-binding protein A Penicillin-Binding Proteins Peptidyl Transferases Phenotype Polymerase Chain Reaction Protein A Proteins Sonication Spores, Bacterial - physiology Sporulation Streptomyces griseus Streptomyces griseus - chemistry Streptomyces griseus - genetics Streptomyces griseus - physiology |
| title | Cloning and characterization of the gene encoding penicillin-binding protein A of Streptomyces griseus |
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