Two forms of DNA-dependent RNA polymerase α subunit in streptomycetes

Abstract We demonstrated two different DNA-dependent RNA polymerase (RNAP) α subunits in spores of Streptomyces granaticolor with apparent molecular masses of 40 and 43 kDa. The 43-kDa subunit was also found in vegetative cells. These two proteins are highly similar to each other as well as to other...

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Published in:FEMS microbiology letters 2000-06, Vol.187 (1), p.9-14
Main Authors: Najmanová, Lucie, Janata, Jiří, Kalousek, František, Novák, Petr, Felsberg, Jürgen, Spížek, Jaroslav
Format: Article
Language:English
Subjects:
C-Terminus
Deoxyribonucleic acid
DNA
DNA-directed RNA polymerase
DNA‐dependent RNA polymerase
E coli
Microbiology
Proteins
Proteolysis
Regulators
Ribonucleic acid
RNA
RNA polymerase
Spores
Streptomyces
Streptomycetes
Transcription
Transcription regulation
Vegetative cells
α Subunit
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container_issue 1
container_start_page 9
container_title FEMS microbiology letters
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creator Najmanová, Lucie
Janata, Jiří
Kalousek, František
Novák, Petr
Felsberg, Jürgen
Spížek, Jaroslav
description Abstract We demonstrated two different DNA-dependent RNA polymerase (RNAP) α subunits in spores of Streptomyces granaticolor with apparent molecular masses of 40 and 43 kDa. The 43-kDa subunit was also found in vegetative cells. These two proteins are highly similar to each other as well as to other bacterial RNAP α subunits. The 40-kDa subunit is shortened from its C-terminus, in the portion of the protein, required for binding of DNA and transcription regulators. The gene for RNAP α from S. granaticolor was cloned and sequenced and the corresponding protein was overproduced in Escherichia coli. In vitro experiments using purified RNAP α showed that the cell free extract from spores of S. granaticolor exhibits proteolytic activity responsible for the α subunit shortening, whereas that from vegetative cells does not. This modification of α subunit might point to a novel mechanism of transcriptional control in streptomycetes.
doi_str_mv 10.1111/j.1574-6968.2000.tb09128.x
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The 43-kDa subunit was also found in vegetative cells. These two proteins are highly similar to each other as well as to other bacterial RNAP α subunits. The 40-kDa subunit is shortened from its C-terminus, in the portion of the protein, required for binding of DNA and transcription regulators. The gene for RNAP α from S. granaticolor was cloned and sequenced and the corresponding protein was overproduced in Escherichia coli. In vitro experiments using purified RNAP α showed that the cell free extract from spores of S. granaticolor exhibits proteolytic activity responsible for the α subunit shortening, whereas that from vegetative cells does not. 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source Oxford Journals Online
subjects C-Terminus
Deoxyribonucleic acid
DNA
DNA-directed RNA polymerase
DNA‐dependent RNA polymerase
E coli
Microbiology
Proteins
Proteolysis
Regulators
Ribonucleic acid
RNA
RNA polymerase
Spores
Streptomyces
Streptomycetes
Transcription
Transcription regulation
Vegetative cells
α Subunit
title Two forms of DNA-dependent RNA polymerase α subunit in streptomycetes
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