Silencing of the Chalcone Synthase Gene by a Virus Vector Derived from the Cucumber Mosaic Virus in Petunia

Virus-induced gene silencing (VIGS) systems are widely used to downregulate target host genes in plants. The Cucumber mosaic virus (CMV), which has a broad host range, has been used as a VIGS vector system in research. In this study, a VIGS vector system derived from the pepo strain of CMV (CMV pepo...

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Bibliographic Details
Published in:Horticulture journal 2019, Vol.88(4), pp.507-513
Main Authors: Tanase, Koji, Matsushita, Yosuke, Mochizuki, Tomofumi
Format: Article
Language:English
Subjects:
Chalcone synthase
flower color
Flowers
Functional analysis
Gene silencing
Host plants
Host range
mRNA
Petunia
Plant viruses
PTGS
VIGS
Viruses
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Summary:Virus-induced gene silencing (VIGS) systems are widely used to downregulate target host genes in plants. The Cucumber mosaic virus (CMV), which has a broad host range, has been used as a VIGS vector system in research. In this study, a VIGS vector system derived from the pepo strain of CMV (CMV pepo) was tested for its use in functional analysis of a target gene in Petunia. The CMV pepo caused systemic infections without severe viral symptoms in petunia plants. A vector containing a short fragment (57 bp) of the chalcone synthase gene (PhCHS-A) was constructed (PhCHSA-CMVpepo) and petunia plants were mechanically inoculated with it. Corollas were white in plants infected with PhCHSA-CMVpepo, but were purple (original color) in plants infected with wild-type CMV pepo. In white corollas, the mRNA level of PhCHS-A was downregulated compared to that in purple corollas. These results indicate that the CMV pepo vector system is useful for VIGS research in flowers of the genera Petunia.
ISSN:2189-0102
2189-0110
DOI:10.2503/hortj.UTD-078