Growth and cytotoxic activity by Mycobacterium ulcerans in protein-free media

Abstract The pathogenic slow-growing Mycobacterium ulcerans has, until now, been cultured in liquid media containing albumin. Here, we report the first description of use of Sauton medium and modified Reid medium, two protein-free media, in which M. ulcerans was able to grow and produce its toxin, a...

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Bibliographic Details
Published in:FEMS microbiology letters 1999-12, Vol.181 (1), p.153-157
Main Authors: Mve-Obiang, Armand, Remacle, José, Palomino, Juan Carlos, Houbion, Andrée, Portaels, Françoise
Format: Article
Language:English
Subjects:
Albumins
Bacterial Toxins - biosynthesis
Bacterial Toxins - toxicity
Bacteriology
Biological and medical sciences
Buruli ulcer
Cell Line
Culture Media - chemistry
Cytotoxicity
Fundamental and applied biological sciences. Psychology
Growth conditions
Growth, nutrition, cell differenciation
Humans
Liquid cultivation
Microbiology
Mycobacterium Infections, Nontuberculous - microbiology
Mycobacterium ulcerans
Mycobacterium ulcerans - growth & development
Mycobacterium ulcerans - metabolism
Proteins
Protein‐free medium
Skin diseases
Toxin activity
Toxins
Virulence
Virulence factors
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Summary:Abstract The pathogenic slow-growing Mycobacterium ulcerans has, until now, been cultured in liquid media containing albumin. Here, we report the first description of use of Sauton medium and modified Reid medium, two protein-free media, in which M. ulcerans was able to grow and produce its toxin, a major virulence factor of this environmental organism which causes a skin disease commonly called Buruli ulcer. These results suggest that Sauton and modified Reid may be useful for certain fields of M. ulcerans research requiring protein-free growth conditions.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1999.tb08838.x