Genetic polymorphism at the β-tubulin locus among human and animal isolates of Cryptosporidium parvum

Abstract Sequence analysis of a fragment of the β-tubulin gene was performed on 13 isolates of the parasite Cryptosporidium parvum, eight from humans and five from animals. A total of 12 synonymous substitutions and a deletion of two bases within the intron sequence were found. This genetic variatio...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 1999-01, Vol.170 (1), p.173-179
Main Authors: Cacciò, Simone, Homan, Wieger, van Dijk, Karin, Pozio, Edoardo
Format: Article
Language:English
Subjects:
Animals
Clonal deletion
Cryptosporidium
Cryptosporidium parvum
Gene deletion
Gene polymorphism
Genetic diversity
Genetic polymorphism
Genotypes
Loci
Microbiology
Multilocus analysis
Parasites
Polymerase chain reaction
Polymorphism
Protozoa
Restriction fragment length polymorphism
Tubulin
β‐Tubulin gene
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Sequence analysis of a fragment of the β-tubulin gene was performed on 13 isolates of the parasite Cryptosporidium parvum, eight from humans and five from animals. A total of 12 synonymous substitutions and a deletion of two bases within the intron sequence were found. This genetic variation defined two alleles at the β-tubulin locus, which can be identified by a simple polymerase chain reaction-restriction fragment length polymorphism assay. A total of 20 isolates were also tested using four available molecular markers. These analyses showed congruently that the C. parvum isolates segregate into two groups, one found exclusively in humans and the other found in both humans and animals. Since no recombinant genotypes were observed, the results are consistent with the hypothesis of a substantially clonal reproduction in this parasite.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1999.tb13371.x