Bioevaluation of 99mTc(I) carbonyl-radiolabeled amino acid coated magnetic nanoparticles in vivo

• Published in: Materials chemistry and physics 2019-09, Vol.235, p.121751, Article 121751
• Main Authors: Büyükok, Onur, Uçar, Eser, İçhedef, Çiğdem, Çetin, Oğuz, Teksöz, Serap
• Format: Article
• Language: English
• Subjects: 99m-technetium tricarbonyl core; Alanine; Amino acids; Carbonyls; Coating; Cysteine; Diagnostic systems; In vivo; Magnetic nanoparticles; Nanoparticles; Radiochemistry; Radiolabeling; Saline solutions; Structural analysis
• ISSN: 0254-0584; 1879-3312
• DOI: 10.1016/j.matchemphys.2019.121751

In this paper, amino acid coated and radiolabeled magnetic nanoparticles (MNPs) were prepared as a potential diagnostic agent. Magnetic nanoparticles were synthesized and functionalized by l-alanine and l-cysteine amino acids in one step. Besides surface functionalization, amino acid coating also provided favourable particle size. Structural characterization was performed by scanning electron microscopy. We obtained 30–38 nm and 22–30 nm diameter ranges with spherical shape for l-alanine and l-cysteine coated nanoparticles, separately. Then l-alanine and l-cysteine coated MNPs were radiolabeled with 99mTc(I)-tricarbonyl core (99mTc(CO)3-Alanine-MNP, 99mTc(CO)3-Cysteine-MNP) with a labeling yield of 63 ± 3% and 74 ± 2%. Pure radiolabeled magnetic particles were obtained by washing them with saline solution, and the radiochemical purity of 99mTc(CO)3-Alanine-MNP and 99mTc(CO)3-Cysteine-MNP were found as 94.3 ± 0.47% and 97.6 ± 1.06% in each of the final solution, respectively. In addition, both radiolabeled nanoparticles were stabile up to 4 h at 37 °C temperature in vitro conditions. After radiolabeling with 99m-Technetium tricarbonyl core, biological behaviour of nanoparticles was evaluated for each in vivo on male Wistar Albino rats. •Radiolabeled nanoparticles gain importance in nanomedicine applications.•Modified iron oxide NP platforms have shown great potential for imaging and therapy.•Aminoacid coated magnetic nanoparticles were synthesized in this work by one step.•Radiolabeling of these magnetic NPs would have implications biological applications.