Sensor and sensor microtiterplate with expanded pH detection range and their use in real samples

•pH indicator with largest range obtained from a single pH probe.•Lanthanide complex indicates pH from pH 2–10.•Sensor membrane works from pH 2–8.•Sensor microtiterplate with quick response from pH 2–8 over 24 h.•pH of real samples from sensor microtiterplate and glass electrode agree well. An impro...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2019-11, Vol.298, p.126848, Article 126848
Main Authors: Al-Qaysi, Wafaa Waleed, Duerkop, Axel
Format: Article
Language:English
Subjects:
Antennas
Dicarboxylic acids
Dynamic range
Emission
Europium
Europium complex
Ligands
Luminescence
pH sensor
Real sample
Sensor microtiterplate
Sensors
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Summary:•pH indicator with largest range obtained from a single pH probe.•Lanthanide complex indicates pH from pH 2–10.•Sensor membrane works from pH 2–8.•Sensor microtiterplate with quick response from pH 2–8 over 24 h.•pH of real samples from sensor microtiterplate and glass electrode agree well. An improved pH indicator derived from a new design concept for luminescent europium complexes and its favourable properties in wide-range pH sensors are introduced. By decorating Eu3+ with ligands that display widespread pKa values and an antenna ligand, this probe covers a dynamic range up to 8 pH units. Unlike wide-range pH sensors that employ multiple organic dyes with multiple pKa values, our concept requires almost no synthetic effort and only one excitation and emission wavelength which simplifies the instrumental requirements. The improved indicator only uses the chelators theonyl trifluoroacetone (TTA) as antenna ligand and pyridine dicarboxylic acid (PDA). The luminescence emission of the Eu3+-TTA-PDA complex in a 1:3:2 feed ratio in aqueous buffer is dependent of pH from pH 2–10. Sensor membranes containing Eu3+-TTA-PDA respond continuously and reversibly from pH 2–8 over 6 pH units in few minutes. This range is one order of magnitude wider than that of a previous lanthanide indicator. Additionally, we fabricated a high-throughput pH sensor microtiterplate with a dynamic range from pH 2–8 over 6 pH units. It constantly works up to 24 h. Finally, we demonstrate that the pH of some real samples taken with the sensor microtiterplate agrees well with the pH obtained by a pH glass electrode.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2019.126848