Efficacy of an artificial microbial siderophore-Fe(III) with high redox potential on correcting Fe chlorosis in rice

• Published in: Soil science and plant nutrition (Tokyo) 2019-09, Vol.65 (5), p.471-478
• Main Authors: Ueno, Daisei, Matsumoto, Kenji, Enami, Takuma, Nishiyama, Nobuo, Kato, Shin-Ichiro, Iwasaki, Kozo
• Format: Article
• Language: English
• Subjects: Alkaline soils; Chelate; Chelates; Chlorophyll; Chlorosis; Deferoxamine; Gene expression; Hydroponics; Iron; Iron deficiency; Microorganisms; Mutants; Nicotianamine; nicotianamine aminotransferase 1; Plant roots; Redox potential; reduction; Rice; Roots; Seedlings; Siderophores; Soil improvement; Soils; Synthesis; uptake
• ISSN: 0038-0768; 1747-0765
• DOI: 10.1080/00380768.2019.1648180

Microbial siderophore-chelated Fe(III) is suggested to be an important source of Fe for plants, although it is hardly reduced by plant roots. Here, we investigated the efficacy of the easily reducible artificial microbial siderophore tris[2-{(N-acetyl-N-hydroxy)glycylamino}ethyl]amine (TAGE)-Fe(III) as an alternative Fe source to correct Fe deficiency in rice plants, and compared it to that of the natural siderophore deferoxamine B (DFOB)-Fe(III). We also evaluated the absorption of Fe from TAGE-Fe(III) by the Strategy I-like system of gramineous plants using nicotianamine aminotransferase 1 (naat1) mutant rice, which does not synthesize phytosiderophores. Fe(III)-siderophores were synthesized in vitro. Nipponbare rice and its naat1 mutant were reared in soil and gel cultures to determine Fe availability. Hydroponically grown naat1 mutant seedlings were used for reducibility assays to determine the ability of rice roots to reduce Fe(III) chelated by TAGE or DFOB. The expression of a Fe-deficiency inducible gene was also determined, as well as chlorophyll and Fe concentrations. Reduci bility assays on naat1 mutant seedlings revealed that the reduction level of TAGE-Fe(III) was approximately three times higher than that of DFOB-Fe(III). Application of TAGE-Fe(III) to both culture medium and alkaline soil improved Fe chlorosis, growth, and Fe concentration in both naat1 and wild type plants, whereas application of DFOB-Fe(III) only did so in wild type plants. Easily reducible Fe(III)-chelates such as TAGE-Fe(III) can be a better source of Fe for rice plants than most natural microbial siderophores-Fe(III). Our study also demonstrated that rice plants have the ability to utilize microbial siderophores-Fe(III) as the Fe source through the Strategy I-like Fe acquisition system.