Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l‐lactic acid)/multi‐walled carbon nanotube scaffolds and naringenin

Spermatogenesis is a process in which animals generate spermatozoa from spermatogonial stem cells (SSCs). Successful in vitro differentiation of SSCs towards spermatids holds a significant promise for regeneration of impaired spermatogenesis. The present study aims to evaluate the efficiency of a 3D...

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Bibliographic Details
Published in:Polymers for advanced technologies 2019-12, Vol.30 (12), p.3011-3025
Main Authors: Ghorbani, Sadegh, Eyni, Hossein, Khosrowpour, Zahra, Salari Asl, Leila, Shabani, Ronak, Nazari, Hojjatollah, Mehdizadeh, Mehdi, Ebrahimi Warkiani, Majid, Amjadi, FatemehSadat
Format: Article
Language:English
Subjects:
Antioxidants
Contact angle
Differentiation
Electric contacts
Electrical resistivity
in vitro spermatogenesis
Lactic acid
Mechanical properties
Multi wall carbon nanotubes
naringenin
PLLA/MWCNTs scaffolds
Polymerase chain reaction
Regeneration
Scaffolds
Spermatogenesis
spermatogonial stem cells (SSCs)
Spermatozoa
Stem cells
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Summary:Spermatogenesis is a process in which animals generate spermatozoa from spermatogonial stem cells (SSCs). Successful in vitro differentiation of SSCs towards spermatids holds a significant promise for regeneration of impaired spermatogenesis. The present study aims to evaluate the efficiency of a 3D culture containing naringenin on proliferation and differentiation potentials of mouse SSCs. In this study, multi‐walled carbon nanotubes (MWCNTs) were incorporated into poly(l‐lactic acid) (PLLA) fibers via electrospinning technique. The fibrous PLLA/MWCNTs were studied by Fourier‐transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), water contact angle measurements, electrical conductivity, and mechanical properties. Next, the SSCs were seeded into the PLLA/MWCNTs scaffolds and exhibited preferable survival and differentiation efficiency to subsequent cell lines. To shed more light on this matter, the immunocytochemistry, reverse‐transcription polymerase chain reaction (RT‐PCR), and qRT‐PCR results showed that the aforementioned cells on the 3D fabrics overexpressed the C‐kit and SYCP3 proteins. In addition, the reactive oxygen species (ROS) measurement data demonstrated that naringenin, an effective antioxidant, plays an important role in in vitro spermatogenesis. Taken together, the results of this study revealed the synergistic effects of 3D scaffolds and naringenin for efficient spermatogenesis in laboratories.
ISSN:1042-7147
1099-1581
DOI:10.1002/pat.4733