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Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l‐lactic acid)/multi‐walled carbon nanotube scaffolds and naringenin
Spermatogenesis is a process in which animals generate spermatozoa from spermatogonial stem cells (SSCs). Successful in vitro differentiation of SSCs towards spermatids holds a significant promise for regeneration of impaired spermatogenesis. The present study aims to evaluate the efficiency of a 3D...
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Published in: | Polymers for advanced technologies 2019-12, Vol.30 (12), p.3011-3025 |
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creator | Ghorbani, Sadegh Eyni, Hossein Khosrowpour, Zahra Salari Asl, Leila Shabani, Ronak Nazari, Hojjatollah Mehdizadeh, Mehdi Ebrahimi Warkiani, Majid Amjadi, FatemehSadat |
description | Spermatogenesis is a process in which animals generate spermatozoa from spermatogonial stem cells (SSCs). Successful in vitro differentiation of SSCs towards spermatids holds a significant promise for regeneration of impaired spermatogenesis. The present study aims to evaluate the efficiency of a 3D culture containing naringenin on proliferation and differentiation potentials of mouse SSCs. In this study, multi‐walled carbon nanotubes (MWCNTs) were incorporated into poly(l‐lactic acid) (PLLA) fibers via electrospinning technique. The fibrous PLLA/MWCNTs were studied by Fourier‐transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), water contact angle measurements, electrical conductivity, and mechanical properties. Next, the SSCs were seeded into the PLLA/MWCNTs scaffolds and exhibited preferable survival and differentiation efficiency to subsequent cell lines. To shed more light on this matter, the immunocytochemistry, reverse‐transcription polymerase chain reaction (RT‐PCR), and qRT‐PCR results showed that the aforementioned cells on the 3D fabrics overexpressed the C‐kit and SYCP3 proteins. In addition, the reactive oxygen species (ROS) measurement data demonstrated that naringenin, an effective antioxidant, plays an important role in in vitro spermatogenesis. Taken together, the results of this study revealed the synergistic effects of 3D scaffolds and naringenin for efficient spermatogenesis in laboratories. |
doi_str_mv | 10.1002/pat.4733 |
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Successful in vitro differentiation of SSCs towards spermatids holds a significant promise for regeneration of impaired spermatogenesis. The present study aims to evaluate the efficiency of a 3D culture containing naringenin on proliferation and differentiation potentials of mouse SSCs. In this study, multi‐walled carbon nanotubes (MWCNTs) were incorporated into poly(l‐lactic acid) (PLLA) fibers via electrospinning technique. The fibrous PLLA/MWCNTs were studied by Fourier‐transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), water contact angle measurements, electrical conductivity, and mechanical properties. Next, the SSCs were seeded into the PLLA/MWCNTs scaffolds and exhibited preferable survival and differentiation efficiency to subsequent cell lines. To shed more light on this matter, the immunocytochemistry, reverse‐transcription polymerase chain reaction (RT‐PCR), and qRT‐PCR results showed that the aforementioned cells on the 3D fabrics overexpressed the C‐kit and SYCP3 proteins. In addition, the reactive oxygen species (ROS) measurement data demonstrated that naringenin, an effective antioxidant, plays an important role in in vitro spermatogenesis. Taken together, the results of this study revealed the synergistic effects of 3D scaffolds and naringenin for efficient spermatogenesis in laboratories.</description><identifier>ISSN: 1042-7147</identifier><identifier>EISSN: 1099-1581</identifier><identifier>DOI: 10.1002/pat.4733</identifier><language>eng</language><publisher>Bognor Regis: Wiley Subscription Services, Inc</publisher><subject>Antioxidants ; Contact angle ; Differentiation ; Electric contacts ; Electrical resistivity ; in vitro spermatogenesis ; Lactic acid ; Mechanical properties ; Multi wall carbon nanotubes ; naringenin ; PLLA/MWCNTs scaffolds ; Polymerase chain reaction ; Regeneration ; Scaffolds ; Spermatogenesis ; spermatogonial stem cells (SSCs) ; Spermatozoa ; Stem cells</subject><ispartof>Polymers for advanced technologies, 2019-12, Vol.30 (12), p.3011-3025</ispartof><rights>2019 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3693-1100ad7e730e63f0a7a37eb7bf959060e8ff448e1289924e2b38cdae644998f93</citedby><cites>FETCH-LOGICAL-c3693-1100ad7e730e63f0a7a37eb7bf959060e8ff448e1289924e2b38cdae644998f93</cites><orcidid>0000-0003-4902-388X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Ghorbani, Sadegh</creatorcontrib><creatorcontrib>Eyni, Hossein</creatorcontrib><creatorcontrib>Khosrowpour, Zahra</creatorcontrib><creatorcontrib>Salari Asl, Leila</creatorcontrib><creatorcontrib>Shabani, Ronak</creatorcontrib><creatorcontrib>Nazari, Hojjatollah</creatorcontrib><creatorcontrib>Mehdizadeh, Mehdi</creatorcontrib><creatorcontrib>Ebrahimi Warkiani, Majid</creatorcontrib><creatorcontrib>Amjadi, FatemehSadat</creatorcontrib><title>Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l‐lactic acid)/multi‐walled carbon nanotube scaffolds and naringenin</title><title>Polymers for advanced technologies</title><description>Spermatogenesis is a process in which animals generate spermatozoa from spermatogonial stem cells (SSCs). Successful in vitro differentiation of SSCs towards spermatids holds a significant promise for regeneration of impaired spermatogenesis. The present study aims to evaluate the efficiency of a 3D culture containing naringenin on proliferation and differentiation potentials of mouse SSCs. In this study, multi‐walled carbon nanotubes (MWCNTs) were incorporated into poly(l‐lactic acid) (PLLA) fibers via electrospinning technique. The fibrous PLLA/MWCNTs were studied by Fourier‐transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), water contact angle measurements, electrical conductivity, and mechanical properties. Next, the SSCs were seeded into the PLLA/MWCNTs scaffolds and exhibited preferable survival and differentiation efficiency to subsequent cell lines. To shed more light on this matter, the immunocytochemistry, reverse‐transcription polymerase chain reaction (RT‐PCR), and qRT‐PCR results showed that the aforementioned cells on the 3D fabrics overexpressed the C‐kit and SYCP3 proteins. In addition, the reactive oxygen species (ROS) measurement data demonstrated that naringenin, an effective antioxidant, plays an important role in in vitro spermatogenesis. Taken together, the results of this study revealed the synergistic effects of 3D scaffolds and naringenin for efficient spermatogenesis in laboratories.</description><subject>Antioxidants</subject><subject>Contact angle</subject><subject>Differentiation</subject><subject>Electric contacts</subject><subject>Electrical resistivity</subject><subject>in vitro spermatogenesis</subject><subject>Lactic acid</subject><subject>Mechanical properties</subject><subject>Multi wall carbon nanotubes</subject><subject>naringenin</subject><subject>PLLA/MWCNTs scaffolds</subject><subject>Polymerase chain reaction</subject><subject>Regeneration</subject><subject>Scaffolds</subject><subject>Spermatogenesis</subject><subject>spermatogonial stem cells (SSCs)</subject><subject>Spermatozoa</subject><subject>Stem cells</subject><issn>1042-7147</issn><issn>1099-1581</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kM9KAzEQxhdRsFbBRwh4qYetyWa72RxL8R8UFKznJZudlJQ0WZNdSm8-gicf0Ccxa_XoaYb5fvMN8yXJJcFTgnF204pumjNKj5IRwZynZFaS46HPs5SRnJ0mZyFsMI4aZ6Pk86UFvxWdW4OFoAPStullp51FTqHwJzqrhUGhgy2SYExAfdB2jaywTunauz6g1pn9xHy9fxgR9yUSUjfXN9vedDoOd8IYaJAUvo7Ww17X14CCFEo50wQkbBPHPrqC1fY8OVHCBLj4rePk9e52tXhIl0_3j4v5MpW04DQl8WXRMGAUQ0EVFkxQBjWrFZ9xXGAolcrzEkhWcp7lkNW0lI2AIs85LxWn4-Tq4Nt699ZD6KqN672NJ6uMkghlRYEjNTlQ0rsQPKiq9Xor_L4iuBpSr2Lq1ZB6RNMDutMG9v9y1fN89cN_A1KAiOI</recordid><startdate>201912</startdate><enddate>201912</enddate><creator>Ghorbani, Sadegh</creator><creator>Eyni, Hossein</creator><creator>Khosrowpour, Zahra</creator><creator>Salari Asl, Leila</creator><creator>Shabani, Ronak</creator><creator>Nazari, Hojjatollah</creator><creator>Mehdizadeh, Mehdi</creator><creator>Ebrahimi Warkiani, Majid</creator><creator>Amjadi, FatemehSadat</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>JG9</scope><orcidid>https://orcid.org/0000-0003-4902-388X</orcidid></search><sort><creationdate>201912</creationdate><title>Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l‐lactic acid)/multi‐walled carbon nanotube scaffolds and naringenin</title><author>Ghorbani, Sadegh ; Eyni, Hossein ; Khosrowpour, Zahra ; Salari Asl, Leila ; Shabani, Ronak ; Nazari, Hojjatollah ; Mehdizadeh, Mehdi ; Ebrahimi Warkiani, Majid ; Amjadi, FatemehSadat</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3693-1100ad7e730e63f0a7a37eb7bf959060e8ff448e1289924e2b38cdae644998f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antioxidants</topic><topic>Contact angle</topic><topic>Differentiation</topic><topic>Electric contacts</topic><topic>Electrical resistivity</topic><topic>in vitro spermatogenesis</topic><topic>Lactic acid</topic><topic>Mechanical properties</topic><topic>Multi wall carbon nanotubes</topic><topic>naringenin</topic><topic>PLLA/MWCNTs scaffolds</topic><topic>Polymerase chain reaction</topic><topic>Regeneration</topic><topic>Scaffolds</topic><topic>Spermatogenesis</topic><topic>spermatogonial stem cells (SSCs)</topic><topic>Spermatozoa</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ghorbani, Sadegh</creatorcontrib><creatorcontrib>Eyni, Hossein</creatorcontrib><creatorcontrib>Khosrowpour, Zahra</creatorcontrib><creatorcontrib>Salari Asl, Leila</creatorcontrib><creatorcontrib>Shabani, Ronak</creatorcontrib><creatorcontrib>Nazari, Hojjatollah</creatorcontrib><creatorcontrib>Mehdizadeh, Mehdi</creatorcontrib><creatorcontrib>Ebrahimi Warkiani, Majid</creatorcontrib><creatorcontrib>Amjadi, FatemehSadat</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Polymers for advanced technologies</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ghorbani, Sadegh</au><au>Eyni, Hossein</au><au>Khosrowpour, Zahra</au><au>Salari Asl, Leila</au><au>Shabani, Ronak</au><au>Nazari, Hojjatollah</au><au>Mehdizadeh, Mehdi</au><au>Ebrahimi Warkiani, Majid</au><au>Amjadi, FatemehSadat</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l‐lactic acid)/multi‐walled carbon nanotube scaffolds and naringenin</atitle><jtitle>Polymers for advanced technologies</jtitle><date>2019-12</date><risdate>2019</risdate><volume>30</volume><issue>12</issue><spage>3011</spage><epage>3025</epage><pages>3011-3025</pages><issn>1042-7147</issn><eissn>1099-1581</eissn><abstract>Spermatogenesis is a process in which animals generate spermatozoa from spermatogonial stem cells (SSCs). 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To shed more light on this matter, the immunocytochemistry, reverse‐transcription polymerase chain reaction (RT‐PCR), and qRT‐PCR results showed that the aforementioned cells on the 3D fabrics overexpressed the C‐kit and SYCP3 proteins. In addition, the reactive oxygen species (ROS) measurement data demonstrated that naringenin, an effective antioxidant, plays an important role in in vitro spermatogenesis. Taken together, the results of this study revealed the synergistic effects of 3D scaffolds and naringenin for efficient spermatogenesis in laboratories.</abstract><cop>Bognor Regis</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1002/pat.4733</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0003-4902-388X</orcidid></addata></record> |
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subjects | Antioxidants Contact angle Differentiation Electric contacts Electrical resistivity in vitro spermatogenesis Lactic acid Mechanical properties Multi wall carbon nanotubes naringenin PLLA/MWCNTs scaffolds Polymerase chain reaction Regeneration Scaffolds Spermatogenesis spermatogonial stem cells (SSCs) Spermatozoa Stem cells |
title | Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l‐lactic acid)/multi‐walled carbon nanotube scaffolds and naringenin |
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