Potential Anticancer Activity of Caspian Cobra Venom Through Induction of Oxidative Stress in Glioblastoma Cell Line

Despite advances in therapeutic strategies in the management of cancer, malignant glioma remains difficult to treat due to progressive resistance to conventional drugs. New studies made efforts to develop new anticancer agents from the screening of natural compounds. The biodiversity of venoms and t...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences, India. Section B: Biological sciences India. Section B: Biological sciences, 2019-12, Vol.89 (4), p.1161-1166
Main Authors: Sinaei, Niloufar, Zare Mirakabadi, Abbas, Behnam, Behzad, Aminzadeh, Azadeh, Karami-Mohajeri, Somayyeh
Format: Article
Language:English
Subjects:
Antitumor activity
Antitumor agents
Behavioral Sciences
Biodiversity
Biomedical and Life Sciences
Cancer
Cell viability
Drug resistance
Glioblastoma
Glioma cells
Life Sciences
Mitochondria
Naja oxiana
Nucleic Acid Chemistry
Oxidative stress
Plant Biochemistry
Reactive oxygen species
Research Article
Venom
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Summary:Despite advances in therapeutic strategies in the management of cancer, malignant glioma remains difficult to treat due to progressive resistance to conventional drugs. New studies made efforts to develop new anticancer agents from the screening of natural compounds. The biodiversity of venoms and their bioactive toxins makes them a special source for the development of novel therapeutic agents. The aim of the present study was to investigate the effect of Naja naja oxiana (NNO) crude venom on U87MG glioma cell line. Cellular viability and the generated amount of reactive oxygen species were determined by MTT and redox-sensitive dye DCFH-DA, respectively. A dose-dependent decline in viability of cells along with increase in generation of reactive oxygen species (ROS) occurred after the 24-h exposure to NNO venom. Incubation of RBC with NNO venom for 24 h indicated that hemolysis was not more than 6%. The results showed that NNO venom might act through the production of excess ROS, further disruption of mitochondrial function, and decrease in viability of U87MG without changes in the integrity of RBC membrane. However, more investigations are needed to find out detailed mechanisms by which NNO venom inhibits the viability of U87MG.
ISSN:0369-8211
2250-1746
DOI:10.1007/s40011-018-1030-9