Chemical derivatization coupled with matrix assisted laser desorption ionization time-of-flight mass spectrometry for convenient and efficient N -glycan detection in human plasma

As one of the most common post-translational modifications, protein N -glycosylation is involved in most important biological processes. Structural changes of the protein linked N -glycans have close correlations with the occurrence and progression of various diseases. Therefore, efficient detection...

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Bibliographic Details
Published in:Analytical methods 2019-12, Vol.11 (46), p.5886-5892
Main Authors: Bai, H. H., Liu, J., Cheng, X. Q., Wang, X. H.
Format: Article
Language:English
Subjects:
Asialofetuin
Biological activity
Biomarkers
Blood plasma
Coupling (molecular)
Diagnostic systems
Glycan
Glycosylation
Hydrophobicity
Ionization
Ions
Mass spectrometry
Mass spectroscopy
N-glycans
Organic chemistry
Polysaccharides
Post-translation
Proteins
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Summary:As one of the most common post-translational modifications, protein N -glycosylation is involved in most important biological processes. Structural changes of the protein linked N -glycans have close correlations with the occurrence and progression of various diseases. Therefore, efficient detection of these disease-associated aberrations is of great significance for the development of new diagnostic biomarkers and therapeutic drug targets. In this work, we developed a method for convenient and effective N -glycan detection by coupling condition controlled chemical derivatization with MALDI-TOF MS. Due to the high reaction efficiency and enhanced hydrophobicity, improved identification scales were obtained for N -glycans from both asialofetuin and human plasma compared with the direct analysis.
ISSN:1759-9660
1759-9679
DOI:10.1039/C9AY01730E