Evaluation of Inoculum Sources for Aerobic Treatment of 2,3,4-Trifluoroaniline During Start-up and Shock

• Published in: Water, air, and soil pollution air, and soil pollution, 2019-12, Vol.230 (12), Article 283
• Main Authors: Zhao, Zhi-Qing, Shen, Xiao-Li, Zheng, Tu-Cai, Abbas, Ghulam, Fan, Rui, Li, Yan-Mei
• Format: Article
• Language: English
• Subjects: Acclimation; Acclimatization; Aerobic treatment; Analysis; Aniline; Atmospheric Protection/Air Quality Control/Air Pollution; Bacteria; Batch reactors; Biodegradation; Bioreactors; Care and treatment; Climate Change/Climate Change Impacts; Defluorination; Drug resistance in microorganisms; Earth and Environmental Science; Environment; Environmental monitoring; Hydrogeology; Industrial parks; Inoculum; Medical wastes; Microorganisms; Municipal wastewater; Next-generation sequencing; Pharmaceutical industry wastes; Purification; Recovery time; Sequencing; Sewage; Shock; Shock resistance; Sludge; Soil Science & Conservation; Stability; Wastewater; Wastewater treatment; Wastewater treatment plants; Water Quality/Water Pollution; Water treatment
• ISSN: 0049-6979; 1573-2932
• DOI: 10.1007/s11270-019-4346-z

Contamination with fluoroaromatics (FAs), particularly polyfluorinated aniline, is becoming a serious environmental problem worldwide. To shorten the start-up time, and increase the stability of treatment systems, this work focused on the effects of three seeding sources on treatment performances of 2,3,4-trifluoroaniline (2,3,4-TFA) during start-up and shock, as well as the acclimated strategy. After 246–323 days of acclimation in a stepwise feeding according to the inhibition degree, three sequencing batch reactors (SBRs) successfully achieved efficient removal, i.e., 300.00 mg/L of 2,3,4-TFA, with over 95.00% of degradation efficiency and 60.00–80.00% of defluorination rates. The sludge obtained from the fluorizated hydrocarbon wastewater treatment plant(FHS) without prior exposure to fluoroaniline was determined to be optimal, based on the observed shortest start-up time of 246 days, the highest defluorination rate of 70.00–80.00%, the fastest recovery time of 7 days after shock, and the highest microbial diversity with nine dominant bacterial groups. Furthermore, compared with the sludge obtained from pharmaceutical wastewater containing part of municipal wastewater treatment plant(PMS), the seeding source used in treating the comprehensive wastewater in industrial park (CIS) exhibited earlier defluorination reaction, higher defluorination rate and microbial diversity, but lower shock resistance. High-throughput sequencing demonstrated that microbial diversity was dependent on the origin of the inoculum after acclimation. We identified two predominant phyla in PMS, namely, Deinococcus-Thermus (24.43%) and Bacteroidetes (18.44%), whereas these were Acidobacteria and Chloroflexi in FHS and CIS. During the shock of 400 mg/L 2,3,4-TFA, the predominant bacteria norank_f_Blastocatellaceae and norank_f_Methylobacteriaceae disappeared, and the defluorination reaction hardly occurred, indicating that the bacterial genera could contribute to the defluorination reaction.