Aggregation and protein corona formation on gold nanoparticles affect viability and liver functions of primary rat hepatocytes

We examined the impact of aggregation and protein corona formation of gold nanoparticles (AuNPs) on the cytotoxicity, uptake and metabolism, specifically urea and albumin synthesis, of primary rat hepatocytes. The AuNPs were synthesized via citrate reduction and the human serum protein corona was pr...

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Bibliographic Details
Published in:Nanomedicine (London, England) England), 2016-09, Vol.11 (17), p.2275-2287
Main Authors: Koh, Wee Ling, Tham, Phoebe Huijun, Yu, Hanry, Leo, Hwa Liang, Yong Kah, James Chen
Format: Article
Language:English
Subjects:
Animals
Cell culture
Cell cycle
Cell Survival - drug effects
Cells, Cultured
Citric Acid - chemistry
Citric Acid - metabolism
Cytotoxicity
Gene expression
Gold - chemistry
Gold - metabolism
gold nanoparticles
Hepatocytes - cytology
Hepatocytes - drug effects
Hepatocytes - metabolism
hepatotoxicity
Liver
liver toxicology
Male
Models, Molecular
Nanoparticles
Nanoparticles - chemistry
Nanoparticles - metabolism
Particle size
primary rat hepatocytes
Protein Corona - metabolism
Proteins
Rats
Rats, Wistar
Rodents
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Summary:We examined the impact of aggregation and protein corona formation of gold nanoparticles (AuNPs) on the cytotoxicity, uptake and metabolism, specifically urea and albumin synthesis, of primary rat hepatocytes. The AuNPs were synthesized via citrate reduction and the human serum protein corona was preformed on the AuNPs. Primary hepatocytes were isolated from male Wistar rats via two-step collagenase perfusion method, and were dosed with both citrate-capped (AuNP-Cit) and protein corona coated AuNPs (AuNP-Cor). The AuNP-Cor showed higher cell uptake and reduced cell viability compared with aggregated AuNP-Cit. Urea and albumin secretions showed AuNP dose dependency. Both AuNP-Cit and AuNP-Cor exerted only an acute effect on the albumin synthesis of hepatocytes with no chronic impact.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm-2016-0173