Loading…

Obtainment and Analysis of Marker-Free Oil Plants Camelina sativa (L.) Expressing of Antimicrobial Peptide Cecropin P1 Gene

Marker-free transgenic Camelina sativa (L.) plants carrying a synthetic gene for cecropin P1, an antimicrobial peptide, under the control of the cauliflower mosaic virus 35S RNA promoter have been obtained and analyzed. The plants were transformed with an agrobacterial binary vector free of selectiv...

Full description

Saved in:
Bibliographic Details
Published in:Applied biochemistry and microbiology 2019-12, Vol.55 (9), p.888-898
Main Authors: Zakharchenko, N. S., Furs, O. V., Pigoleva, S. V., Dyachenko, O. V., Aripovskii, A. V., Buryanov, Ya. I., Shevchuk, T. V.
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Marker-free transgenic Camelina sativa (L.) plants carrying a synthetic gene for cecropin P1, an antimicrobial peptide, under the control of the cauliflower mosaic virus 35S RNA promoter have been obtained and analyzed. The plants were transformed with an agrobacterial binary vector free of selective genes of antibiotic and herbicide resistance. The marker-free transformants were screened via measurement of the antibacterial activity of cecropin P1 and enzyme immunoassay. The obtained plants exhibited an increased resistance to infection with the bacteria Erwinia carotovora , the fungi Fusarium graminearum , and oxidative stress during infection. Analysis of the fatty acid composition of seed oil showed an increased amount of α-linolenic acid in the transgenic Camelina lines as compared to unmodified plants. The results indicate that the cecropin P1 gene can be included in an integral antistress plant-protective system.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683819090096