Proximate Mechanisms of Host Plant Location by a Specialist Phytophagous Insect, the Grape Berry Moth, Paralobesia Viteana

• Published in: Journal of chemical ecology 2019-12, Vol.45 (11-12), p.946-958
• Main Authors: Wolfin, Michael S., Chilson, Ronald R., Thrall, Jonathan, Liu, Yuxi, Volo, Sara, Cha, Dong H., Loeb, Gregory M., Linn, Charles E.
• Format: Article
• Language: English
• Subjects: Agriculture; Animals; Behavior, Animal; Biochemistry; Biological Microscopy; Biomedical and Life Sciences; Butterflies & moths; Chromatography; Cornus - chemistry; Ecology; Electroantennograms; Entomology; Female; Females; Flight; Flight tunnel; Fruits; Gas chromatography; Gas Chromatography-Mass Spectrometry - methods; Grapes; Host plants; Host Specificity; Host-Parasite Interactions; Insect Control - methods; Insects; Landing; Landing behavior; Life Sciences; Malus - chemistry; Mass spectrometry; Mass spectroscopy; Mixtures; Moths - physiology; Odorants - analysis; Olfactory discrimination; Olfactory discrimination learning; Oviposition - drug effects; Plant extracts; Plant Extracts - chemistry; Plant location; Plant Shoots - chemistry; Shoots; Smell; Vitis - chemistry; Vitis - parasitology; Volatile compounds; Volatile Organic Compounds - chemistry; Volatile Organic Compounds - metabolism; Volatiles
• ISSN: 0098-0331; 1573-1561
• DOI: 10.1007/s10886-019-01112-1

There are contrasting hypotheses regarding the role of plant volatiles in host plant location. We used the grape berry moth (GBM; Paralobesia viteana )-grape plant ( Vitis spp. ) complex as a model for studying the proximate mechanisms of long distance olfactory-mediated, host-plant location and selection by a specialist phytophagous insect. We used flight tunnel assays to observe GBM female in-flight responses to host ( V. riparia ) and non-host (apple, Malus domestica ; and gray dogwood, Cornus racimosa ,) odor sources in the form of plant shoots, extracts of shoots, and synthetic blends. Gas chromatography-electroantennographic detection and gas chromatography/mass spectrometry analyses were used to identify antennal-active volatile compounds. All antennal-active compounds found in grape shoots were also present in dogwood and apple shoots. Female GBM flew upwind to host and non-host extracts and synthetic blends at similar levels, suggesting discrimination is not occurring at long distance from the plant. Further, females did not land on sources releasing plant extracts and synthetic blends, suggesting not all landing cues were present. Additionally, mated and unmated moths displayed similar levels of upwind flight responses to all odor sources, supporting the idea that plant volatiles are not functioning solely as ovipositional cues. The results of this study support a hypothesis that GBM females are using volatile blends to locate a favorable habitat rather than a specific host plant, and that discrimination is occurring within the habitat, or even post-landing.