Sclerotinia Sclerotiorum (White Mold): Cytotoxic, Mutagenic, and Antimalarial Effects In Vivo and In Vitro

• Published in: Journal of food science 2019-12, Vol.84 (12), p.3866-3875
• Main Authors: Pressete, Carolina Girotto, Giannini, Laila Santos Vieira, Paula, Daniela Aparecida Chagas, do Carmo, Mariana Araújo Vieira, Assis, Diego Magno, Santos, Mário Ferreira Conceição, Machado, José da Cruz, Marques, Marcos José, Soares, Marisi Gomes, Azevedo, Luciana
• Format: Article
• Language: English
• Subjects: Antimalarials - analysis; Antimalarials - isolation & purification; Antimalarials - pharmacology; apoptosis; Ascomycota - chemistry; Biocompatibility; Biological effects; Biological Products - analysis; Biological Products - isolation & purification; Biological Products - pharmacology; Biomarkers; Cancer; Cell Line, Tumor; Cell Survival - drug effects; Cells, Cultured; Chromatography, High Pressure Liquid; Colon; Contamination; Cytotoxicity; Deoxyribonucleic acid; DNA; Erythrocytes; fungus; Genotoxicity; Health risks; Hepatocytes; Humans; In vivo methods and tests; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipids; Liquid chromatography; Malaria; Mass Spectrometry; Mass spectroscopy; Mutagenesis; mutagenicity; Mutagens - analysis; Mutagens - isolation & purification; Mutagens - pharmacology; Organic chemistry; Oxidative stress; Peripheral blood; Peroxidation; Reactive oxygen species; Reactive Oxygen Species - analysis; Reactive Oxygen Species - metabolism; Sclerotia; Toxicity; Vector-borne diseases; White mold
• ISSN: 0022-1147; 1750-3841
• DOI: 10.1111/1750-3841.14910

This work aimed includes performing the sclerotia chemical profile and evaluates their biological effects on mutagenesis, oxidative stress, cancer, and malaria. A chemical profile was determined by ultraperformance liquid chromatography mass spectrometry (UHPLC–HRMS) analysis dereplicating norditerpenoid dilactone, sclerolide, and other compounds. The GI50 values to cancer cells (19.8 to 277.6 µg/mL) were higher than normal (16.05 µg/mL), meaning high cytotoxicity. Regarding the oxidative stress, the results showed that the all AcOET fraction concentrations tested on IMR90 noncancer cell increased reactive oxygen species (ROS) production in more intense way (by fivefold) than in tested cancer cells. The in vivo study showed an increase of the following biomarkers (by 296.00%): % DNA in comet tail in peripheral blood and liver cells; micronucleated erythrocytes and colon cells and lipid serum peroxidation. These results indicate the sclerotia as genotoxic and mutagenic agent and its contamination may lead to fungal toxic effects with a risk to human health.