Copper treatment alters the permeability of tight junctions in cultured human intestinal Caco-2 cells

The effects of copper on tight-junction permeability were investigated in human intestinal Caco-2 cells, monitoring transepithelial electrical resistance and transepithelial passage of mannitol. Apical treatment of Caco-2 cells with 10-100 muM CuCl2 (up to 3 h) produced a time- and concentration-dep...

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Bibliographic Details
Published in:American journal of physiology: Gastrointestinal and liver physiology 1999-12, Vol.40 (6), p.G1138-G1148
Main Authors: FERRUZZA, S, SCARINO, M.-L, ROTILIO, G, CIRIOLO, M. R, SANTARONI, P, MUDA, A. O, SAMBUY, Y
Format: Article
Language:English
Subjects:
Biological and medical sciences
Copper
Digestive system
Food toxicology
Medical sciences
Proteins
Toxicology
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Summary:The effects of copper on tight-junction permeability were investigated in human intestinal Caco-2 cells, monitoring transepithelial electrical resistance and transepithelial passage of mannitol. Apical treatment of Caco-2 cells with 10-100 muM CuCl2 (up to 3 h) produced a time- and concentration-dependent increase in tight-junction permeability, reversible after 24 h in complete medium in the absence of added copper.
ISSN:0193-1857
1522-1547