Estimation of Nateglinide by using MBTH as a Chromogenic Reagent

Invitro pharmacological studies suggest that nateglinide stimulates insulin secretion by blocking ATP sensitive potassium channel in pancreatic beta-cells, causing cell membrane depolarization which results in calcium influx and insulin secretion1-3. There were no visible spectrophotometric method h...

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Bibliographic Details
Published in:Asian Journal of Pharmaceutical Analysis 2019-12, Vol.9 (4), p.199
Main Authors: Babu, N. Raghavendra, Padmavathi, Y., Babu, R. Swethan, Unnisa, Amreen, Bhavana, M., Kumar, P. Ravi
Format: Article
Language:English
Subjects:
Chemistry
Chloride
Diabetes
Drug dosages
Insulin
Methods
Pharmaceutical industry
Pharmaceuticals
Pharmacy
Scientific imaging
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Summary:Invitro pharmacological studies suggest that nateglinide stimulates insulin secretion by blocking ATP sensitive potassium channel in pancreatic beta-cells, causing cell membrane depolarization which results in calcium influx and insulin secretion1-3. There were no visible spectrophotometric method has been developed, which was simple and economical when compared to other UV methods. [...]the present study includes development of fast, accurate and sensitive visible spectrophotometric method for the estimation of nateglinide as an active pharmaceutical ingredient as well as in pharmaceutical formulation by using MBTH as a chromogenic reagent. Optimum conditions and Spectral data max 634nm Colour Apple Green Beer's law range 50-300g/mL Sandell's sensitivity (g/cm2/0.001 absorbance units) 3.98 Molar absorptivity (litre/moles/cm) 1.75x10-3 Limit of detection (g/mL) 11.78 Limit of quantification (g/mL) 35.7 Regression equation (y) y=0.002x-0.046 Slope (a) 0.002 Intercept (c) -0.046 Correlation coefficient (r2) 0.998 y= mx+c where c is the concentration of drug in g/ml and y is the absorbance measured at max. Inter-day precision data for nateglinide at 634nm using MBTH Reagent S. No Concentration (g/ml)) Mean Absorbance* Mean Standard deviation % RSD 1 120 Morning 0.210 0.212 0.002 1.18 Afternoon 0.212 Evening 0.215 2 150 Morning 0.238 0.241 0.004 1.66 Afternoon 0.243 Evening 0.246 3 180 Morning 0.295 0.304 0.005 1.70 Afternoon 0.302 Evening 0.305 *Average of 3 determinations The results shows that all the calculated % RSD values for both interday and intraday precision are below 2, therefore the method is precise.
ISSN:2231-5667
2231-5675
DOI:10.5958/2231-5675.2019.00033.4