Use of EPR spectroscopy to check the changes in organism radioresistance. Experimental results

The responses of dNTP, DNA, and protein synthesis systems in blood-forming organs of animals (dogs, mice) as well as changes in blood Fe 3+ -transferrin (Fe-TF) and Cu 2+ -ceruloplasmin (Cu-CP) pools upon γ-irradiation and administration of radioprotectors have been studied. It is shown that changes...

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Bibliographic Details
Published in:Biophysics (Oxford) 2009-04, Vol.54 (2), p.214-222
Main Authors: Sharygin, V. L., Pulatova, M. K., Shlyakova, T. G., Todorov, I. N., Mitrokhin, Yu. I., Wasserman, A. M., Yordanov, N. D.
Format: Article
Language:English
Subjects:
Biological and Medical Physics
Biophysics
Complex Systems Biophysics
Deoxyribonucleic acid
DNA
Dogs
Enzymes
Physics
Physics and Astronomy
Protein synthesis
Spectrum analysis
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Summary:The responses of dNTP, DNA, and protein synthesis systems in blood-forming organs of animals (dogs, mice) as well as changes in blood Fe 3+ -transferrin (Fe-TF) and Cu 2+ -ceruloplasmin (Cu-CP) pools upon γ-irradiation and administration of radioprotectors have been studied. It is shown that changes in Fe-TF and Cu-CP pools are indices of change in body radioresistance and are reliably checked by the EPR technique. An increase in the Fe-TF pool promotes the activation of synthesis of dNTP, DNA, and Fe 3+ -containing proteins, which are essential for repair efficiency during early postirradiation time as well as for the development of compensatory-restorative reactions of cellular systems; i.e., they are responsible for body resistance to DNA-damaging factors. It is important that the intensity of responses depends on the initial state of the organism. Thus, dogs with initial individual characteristics of blood typical of “depressed” or “activated” states had abnormally high responses to irradiation at low doses of 0.25 and 0.5 Gy. This fact is important for estimating the consequences of prolonged low-dose irradiation for the human population. It has been shown that radioprotectors efficient in the survival test activate the synthesis of dNTP, DNA, and proteins in organs. The intensity of dNTP synthesis and the time when dNTP pools become maximal determine the efficiency of protectors and the time of irradiation after their administration.
ISSN:0006-3509
1555-6654
DOI:10.1134/S0006350909020171