Computer simulation of plastocyanin-cytochrome f complex formation in the thylakoid lumen

Plastocyanin diffusion in the thylakoid lumen and its binding to cytochrome f (a subunit of the membrane b 6 f complex) were studied with a direct multiparticle simulation model that could also take account of their electrostatic interaction. Experimental data were used to estimate the model paramet...

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Bibliographic Details
Published in:Biophysics (Oxford) 2008-04, Vol.53 (2), p.140-146
Main Authors: Kovalenko, I. B., Abaturova, A. M., Gromov, P. A., Ustinin, D. M., Riznichenko, G. Yu, Grachev, E. A., Rubin, A. B.
Format: Article
Language:English
Subjects:
Biological and Medical Physics
Biophysics
Cell Biophysics
Physics
Physics and Astronomy
Plant sciences
Proteins
Simulation
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Summary:Plastocyanin diffusion in the thylakoid lumen and its binding to cytochrome f (a subunit of the membrane b 6 f complex) were studied with a direct multiparticle simulation model that could also take account of their electrostatic interaction. Experimental data were used to estimate the model parameters for plastocyanin-cytochrome f complexing in solution. The model was then employed to assess the dependence of the association rate constant on the dimensions of the lumen. Highest rates were obtained at a lumen span of 8–10 nm; narrowing of the lumen below 7 nm resulted in drastic deceleration of complexing. This corresponded to the experimentally observed effect of hyperosmotic stress on the interaction between plastocyanin and cytochrome f in thylakoids.
ISSN:0006-3509
1555-6654
DOI:10.1134/S0006350908020048