Preparation and preliminary evaluation of bio-nanocomposites based on hydroxyapatites with antibacterial properties against anaerobic bacteria

• Published in: Materials Science & Engineering C 2020-01, Vol.106, p.110295, Article 110295
• Main Authors: Zawisza, Katarzyna, Sobierajska, Paulina, Nowak, Nicole, Kedziora, Anna, Korzekwa, Kamila, Pozniak, Blazej, Tikhomirov, Marta, Miller, Julia, Mrowczynska, Lucyna, Wiglusz, Rafal J.
• Format: Article
• Language: English
• Subjects: Adsorption; Anaerobic bacteria; Animals; Antibacterial effects; Antimicrobial activity; Antimicrobial agents; Bacteria; Bacteroidetes - drug effects; Biocompatibility; Biocompatible Materials - chemical synthesis; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Biomedical materials; Biotechnology; Blood Sedimentation - drug effects; Calcium hydroxyapatite nanopowders; Cattle; Cell Line, Tumor; Cell Survival - drug effects; Chemical synthesis; Cytotoxicity; Electron microscopy; Emission spectra; Erythrocytes; Europium; Europium - chemistry; Europium and silver ions; Excitation spectra; Extracellular matrix; Haemolytic activity; Hemolysis - drug effects; Humans; Hydroxyapatite; Hydroxyapatites - chemistry; Infrared spectroscopy; Materials science; Metronidazole; Microbial Sensitivity Tests; Multifunctional materials; Muramidase - chemistry; Nanocomposites; Nanocomposites - chemistry; Nanocomposites - toxicity; Nitroimidazole; Organic chemistry; Osteosarcoma; Photoluminescence; Photons; Prevotella - drug effects; Properties (attributes); Raman spectroscopy; Serum Albumin, Bovine - chemistry; Silver - chemistry; Tinidazole; Toxicity; X-ray diffraction
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2019.110295

Hexagonal nanocrystalline powders of the non-doped Ca10(PO4)6(OH)2 as well as activated with Ag+ and Eu3+ ions were synthesized by using different wet chemistry methods. Moreover, the obtained hydroxyapatite was loaded with Ag0, as well as nitroimidazole antimicrobials: metronidazole and tinidazole. The structural properties of the products were analyzed by X-ray diffraction (XRD), scanning (SEM) and transmission (TEM) electron microscopy as well as infrared (IR) and Raman spectroscopy. The photoluminescence properties of the Eu3+ and Ag+ co-doped Ca10(PO4)6(OH)2 were characterized via the PL emission, excitation spectra and the luminescence decay curve. The antimicrobial activity of the obtained materials against Prevotella bivia and Parabacteroides distasonis was studied. The cytotoxicity assessment was carried out on the human osteosarcoma cell line (U2OS) as well as human red blood cells (RBC). The choice of the in vitro model was based on the fact that U2OS is a cancer cell line derived from bone tissue which is rich in apatites that play a pivotal role in the extracellular matrix formation. RBCs are the most abundant blood cells and they are used as a cell model in the study of biocompatibility of new prepared biocompounds with potential medical applications. The obtained multifunctional materials do not exhibit the haemolytic activity, therefore, they could be used as a promising antimicrobial agent and for anaerobic bacteria. •Nanocrystalline non-doped Ca10(PO4)6(OH)2 and activated with Ag+ and Eu3+ ions were synthesized.•The obtained hydroxyapatite was loaded with Ag0 and nitroimidazole antimicrobials.•The antimicrobial activity of the obtained materials against Prevotella bivia and Parabacteroides distasonis was studied.•The cytotoxicity was carried out on the human osteosarcoma cell line (U2OS) and human red blood cells (RBC).