Biosynthesis of silver and gold nanoparticles using Musa acuminata colla flower and its pharmaceutical activity against bacteria and anticancer efficacy

• Published in: Journal of photochemistry and photobiology. B, Biology Biology, 2019-12, Vol.201, p.111670, Article 111670
• Main Authors: Valsalam, Saritha, Agastian, Paul, Esmail, Galal Ali, Ghilan, Abdul-Kareem Mohammed, Al-Dhabi, Naif Abdullah, Arasu, Mariadhas Valan
• Format: Article
• Language: English
• Subjects: Antibacterial activity; Anticancer; Anticancer properties; Antioxidants; Bacteria; Biosynthesis; Cancer; Ethanol; Flowers; FTIR; GC–MS; Gold; Gold nanoparticles; In vitro methods and tests; Ions; Musa acuminata; Musa acuminata colla; Nanoparticles; Organic chemistry; Palmitic acid; Pharmaceuticals; Planes; Reducing agents; Silver; Toxicity; UV - visible spectrum; Vero cells; Visible spectrum; XRD; β Lactamase
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2019.111670

Synthesis of nanoparticles using plant sources as reducing agent has become important, as physical and chemical methods are costlier and affects environment. Hence it is important to develop environment friendly nanoparticle synthesis by avoiding the use of toxic chemicals. The present study aimed to synthesize silver nanoparticles (Ag Nps) and gold nanoparticles (AuNps) using Musa acuminata colla flower and its pharmaceutical activity against extended spectrum beta-lactamase (ESBL) gene producing bacteria and anticancer efficacy. The synthesized Ag and Au NPs were analysed by means of UV–Vis, FTIR, XRD,SEM and EDAX evidenced the bioreduction of Ag+ ions to Ag0 and Au3+ ions to Au0 respectively. Both nanoparticles and flower extracts were studied for antibacterial activity of ESBL gene producing bacteria by disc diffusion and microdilution (Resazurin) method. In vitro anticancer efficacy (MCF-7) and toxicity (VERO) of AgNPs, AuNPs, aqueous extract and ethanol extract of flowers were performed by MTT assay. IC50 value for DPPH analysis was at 390 μg and 460 μg for ethanol and aqueous extract respectively. Total antioxidant content was found be 740 μg/mg and 460 μg/mg for ethanol and aqueous extract. GCMS analysis authenticated the existence of the compounds namely, 9,12-octadecadienoic acid(z,z)- and n-hexadecanoic acid in the crude extract of the samples. Among the samples, AgNPs had best antibacterial activity. AgNPs and AuNPs were confirmed by colour change to reddish brown and ruby red. Further ƛmax were obtained at 474 and 540 nm by UV - visible spectrum. SEM analysis revealed the particle size ranges from 12.6 to 15.7 nm for silver and 10.1 to 15.6 nm for gold nanoparticles. The EDAX spectrum shows a strong signal for elemental Ag and Au at ~ 3 keV and 1.5 keV. The XRD patterns for silver and gold nanoparticles at 36.701, 42.900, 63.281 and 76.398 corresponding to the lattice planes 2.4467, 2.1064, 1.46839, 1.24564 nm and 27.32, 36.7228, 39.56, 42.888, 63.253, 63.253, 65.02 and 76.383 corresponding to the lattice planes 3.262, 2.44530, 2.276, 2.1070, 1.46897, 1.4332 and 1.24585 nm. The IC50 values for MCF-7 and VERO cells were 30.0 μg/ml and 55.0 μg/ml respectively. •Ag and Au nanoparticles were green synthesized from the flower extract of M.acuminata colla.•Nanoparticles showed maximum absorption at 474 nm and 540 nm.•TEM and SEM analysis confirmed the nanoparticels were lattice plane.•Nanoparticles exhibited activity against Pseudomonas aeroginosa.•