Detection of microorganism from China's spacecraft assembly cleanroom

Adequate research showed that microorganisms are ubiquitous in orbiting space station, which have a potential impact on the health status of astronauts and reliability of equipment. Microbial community analysis of spacecraft-associated assembly cleanroom is important, since they are considered as pr...

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Bibliographic Details
Published in:Acta astronautica 2020-01, Vol.166, p.545-547
Main Authors: Zhang, Ying, Xin, Cong-xin, Wang, Xiang, Deng, Yu-Lin
Format: Article
Language:English
Subjects:
Aerosol fungi
Air sampling
Assembly
Astronauts
Bacteria
China'S space station
Chinese spacecraft
Cleanrooms
Contact plate
Fungi
Fungi in air
Microorganisms
Quantitative loop-mediated isothermal amplification (qLAMP)
Reliability analysis
Space stations
Spacecraft
Spacecraft equipment
Spacecraft reliability
Viability
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Summary:Adequate research showed that microorganisms are ubiquitous in orbiting space station, which have a potential impact on the health status of astronauts and reliability of equipment. Microbial community analysis of spacecraft-associated assembly cleanroom is important, since they are considered as prime culprit by some to cause spacecraft equipment malfunction. In this study, culturable bacteira in spacecraft assembly cleanroom (SAC) was detected by contact plate. Furthermore, quantitative loop-mediated isothermal amplification (qLAMP) was first used to assess the abundance of fungi in air sample of SAC. Thirty eight bacteria were isolated and identified. The total culturable bacterial burden for SAC surface samples was 36–632 CFU/100 m2, while fungal burden was 160–236 CFU/100 m2. The fungal burden for SAC air samples was 5.36 × 102–3.62 × 104 28S rRNA gene copies/m3. A viability ratio of 12.36–23.12% was detected by PMA treatment. The results of this study first revealed the overall culturable bacteria diversity and fungal abundance in the SAC of China, which will facilitate further development of a targeted biological safety prevention and maintenance practices. •Microorganism in China's spacecraft assembly cleanroom (SAC) was first studied.•Bacillus, Staphylococcus and Micrococcus are key strains for prevention and control.•The qLAMP assay was proved to be sensitive and suitable for analyzing SAC samples.•PMA treatment is important when determining biocontamination in SAC samples.
ISSN:0094-5765
1879-2030
DOI:10.1016/j.actaastro.2018.08.024