Hypoxia‐sensitive LINC 01436 is regulated by E2F6 and acts as an oncogene by targeting miR‐30a‐3p in non‐small cell lung cancer

Dysregulation of long noncoding RNA (lncRNA) is known to be involved in numerous human diseases, including lung cancer. However, the precise biological functions of most lnc RNA remain to be elucidated. Here, we identified a novel up‐regulated lnc RNA , LINC 01436 (RefSeq: NR _110419.1), in non‐smal...

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Bibliographic Details
Published in:Molecular oncology 2019-04, Vol.13 (4), p.840-856
Main Authors: Yuan, Shuai, Xiang, Ying, Wang, Guilu, Zhou, Meiyu, Meng, Gang, Liu, Qingyun, Hu, Zeyao, Li, Chengying, Xie, Weijia, Wu, Na, Wu, Long, Cai, Tongjian, Ma, Xiangyu, Zhang, Yao, Yu, Zubin, Bai, Li, Li, Yafei
Format: Article
Language:English
Subjects:
Binding sites
Cancer therapies
Cell adhesion & migration
Cell cycle
Cell migration
Genes
Hypoxia
Lung cancer
Lung diseases
Medical prognosis
Metastases
Metastasis
MicroRNAs
miRNA
Non-small cell lung carcinoma
Oncogenes
Plasmids
Proteins
Small cell lung carcinoma
Transcription
Tumors
Xenografts
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Summary:Dysregulation of long noncoding RNA (lncRNA) is known to be involved in numerous human diseases, including lung cancer. However, the precise biological functions of most lnc RNA remain to be elucidated. Here, we identified a novel up‐regulated lnc RNA , LINC 01436 (RefSeq: NR _110419.1), in non‐small cell lung cancer ( NSCLC ). High expression of LINC 01436 was significantly associated with poor overall survival. Notably, LINC 01436 expression was transcriptionally repressed by E2F6 under normoxia, and the inhibitory effect was relieved in a hypoxic microenvironment. Gain‐ and loss‐of‐function studies revealed that LINC 01436 acted as a proto‐oncogene by promoting lung cancer cell growth, migration and invasion in vitro . Xenograft tumor assays in nude mice confirmed that LINC 01436 promoted tumor growth and metastasis in vivo . Mechanistically, LINC 01436 exerted biological functions by acting as a microRNA (miR)‐30a‐3p sponge to regulate the expression of its target gene EPAS 1 . Our findings characterize LINC 01436 as a new hypoxia‐sensitive lnc RNA with oncogenic function in NSCLC , suggesting that LINC 01436 may be a potential biomarker for prognosis and a potential target for treatment.
ISSN:1574-7891
1878-0261
DOI:10.1002/1878-0261.12437