ORF YBL042 of Saccharomyces cerevisiae encodes a uridine permease

The purpose of this work was to identify the function of an open reading frame called YBL042, found during the systematic sequencing of Saccharomyces cerevisiae's chromosome II. The YBL042 gene product shows 70% similarity with the uracil permease and the allantoin permease encoded by FUR4 and...

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Bibliographic Details
Published in:FEMS microbiology letters 1998-02, Vol.159 (1), p.69-75
Main Authors: Wagner, R, Montigny, J. de, Wergifosse, P. de, Souciet, J.L, Potier, S
Format: Article
Language:English
Subjects:
Allantoin
Allantoin - metabolism
Allantoin permease
Alleles
Biological and medical sciences
Biological Transport
Chromosome 2
Chromosomes
Chromosomes, Fungal - genetics
Cloning, Molecular
Disruption
FUI1
FUI1 gene
Fundamental and applied biological sciences. Psychology
Fungi
Gene Dosage
Gene sequencing
Genes, Fungal - genetics
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Membrane Transport Proteins - genetics
Microbiology
Mutation
Mycology
Open Reading Frames
Permease
Phenotypes
Pyrimidines
Saccharomyces cerevisiae
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Transport
Uracil
Uracil - metabolism
Uracil permease
Uridine
Uridine permease
Yeast
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Summary:The purpose of this work was to identify the function of an open reading frame called YBL042, found during the systematic sequencing of Saccharomyces cerevisiae's chromosome II. The YBL042 gene product shows 70% similarity with the uracil permease and the allantoin permease encoded by FUR4 and DAL4, respectively. The mutation constructed by disruption of this ORF is allelic to the FUI1 gene previously described as encoding the uridine permease but not cloned yet. A strain carrying the disrupted allele and a fui1 mutant exhibit the same phenotype as they do not grow on a medium containing uridine as the sole source of pyrimidines and as they are resistant to 10(-3) M 5-fluorouridine (5FUI), a toxic analog of uridine. Even though the FUI1 gene has a multicopy suppressor effect on uracil transport, its product does not seem to be involved in this transport, in contrast to the FUR4 gene product which is involved in uridine transport. Moreover, the FUI1 gene product does not play any role in allantoin transport.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1998.tb12843.x