Cloning and characterization of the recA gene of Paracoccus denitrificans and construction of a recA-deficient mutant

Abstract The recA gene of Paracoccus denitrificans has been isolated from a genomic library by hybridization with the Rhodobacter sphaeroides recA gene. Its complete nucleotide sequence consists of 1071 bp encoding a polypeptide of 356 amino acids. Nucleotide sequence analysis of the P. denitrifican...

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Bibliographic Details
Published in:FEMS microbiology letters 1997-02, Vol.147 (2), p.209-213
Main Authors: Fernandez de Henestrosa, Antonio R, del Rey, Alfonso, Tarragó, Raül, Barbé, Jordi
Format: Article
Language:English
Subjects:
Amino acids
Bacteria
Cloning
Defective mutant
Deficient mutant
Deoxyribonucleic acid
DNA
DNA damage
Genes
Hybridization
Microbiology
Nucleotide sequence
Nucleotides
Paracoccus denitrificans
Phototrophic bacteria
Polypeptides
RecA protein
Regulatory sequences
Sequence analysis
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Summary:Abstract The recA gene of Paracoccus denitrificans has been isolated from a genomic library by hybridization with the Rhodobacter sphaeroides recA gene. Its complete nucleotide sequence consists of 1071 bp encoding a polypeptide of 356 amino acids. Nucleotide sequence analysis of the P. denitrificans recA gene revealed the closest identities with the R. sphaeroides and the Rhodobacter capsulatus recA genes. Nevertheless, and surprisingly, recA genes of these two phototrophic bacteria are not DNA damage-inducible when introduced into P. denitrificans cells, whereas recA genes of both P. denitrificans and Rhizobium etli are. These results suggest that the promoters of P. denitrificans and R. etli recA genes have a similar regulatory sequence. A recA-defective mutant of P. denitrificans has also been constructed by replacement of the active recA gene by an in vitro inactivated gene copy.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1997.tb10243.x