Loading…
Ammonium addition inhibits 13C-methane incorporation into methanotroph membrane lipids in a freshwater sediment
Abstract To investigate the effect of ammonium addition on the species composition and activity of freshwater methane oxidizing bacteria, intact sediment cores were labeled with 13CH4 and incubated under ambient and elevated ammonium concentrations. After 7 days, methanotroph activity was assessed b...
Saved in:
| Published in: | FEMS microbiology ecology 1999-05, Vol.29 (1), p.81-89 |
|---|---|
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c3171-bf479724bdfa686fb79c949bb4372d3f30c64cb3f1447cbe4d7b1d86befba4f43 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c3171-bf479724bdfa686fb79c949bb4372d3f30c64cb3f1447cbe4d7b1d86befba4f43 |
| container_end_page | 89 |
| container_issue | 1 |
| container_start_page | 81 |
| container_title | FEMS microbiology ecology |
| container_volume | 29 |
| creator | Nold, Stephen C. Boschker, Henricus T.S. Pel, Roel Laanbroek, Hendrikus J. |
| description | Abstract
To investigate the effect of ammonium addition on the species composition and activity of freshwater methane oxidizing bacteria, intact sediment cores were labeled with 13CH4 and incubated under ambient and elevated ammonium concentrations. After 7 days, methanotroph activity was assessed by quantifying the isotopic composition of the carbon in membrane lipids. The 16-carbon rather than the 18-carbon methanotroph-specific biomarkers showed a clear enrichment in 13C, suggesting the importance of group I methanotrophs in these sediments. Ammonium addition resulted in a depleted isotopic signal compared to ambient controls, suggesting that high ammonium concentrations inhibit methane incorporation into cellular components. These results compare favorably with studies that showed ammonium inhibition of methane oxidation, and extend these findings by demonstrating the effect of nitrogen fertilization on methanotroph lipid synthesis. |
| doi_str_mv | 10.1111/j.1574-6941.1999.tb00600.x |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2331813876</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1111/j.1574-6941.1999.tb00600.x</oup_id><sourcerecordid>2331813876</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3171-bf479724bdfa686fb79c949bb4372d3f30c64cb3f1447cbe4d7b1d86befba4f43</originalsourceid><addsrcrecordid>eNqVkM1OAyEUhYnRxFp9h4m6nREKhcHERdP4l2jc6JoAAylNZxiBpvr2MplGN7qQDeHe79xzOQCcI1ihfK7WFZozUlJOUIU451VSEFIIq48DMPluHYIJRLQuKeH0GJzEuIYQzTGBE-AXbes7t20L2TQuOd8Vrls55VIsEF6WrUkr2Zlc1D70Psg9knwxtnwKvl_lR6vCAG5c75qYiUIWNpi42slkQhFN41rTpVNwZOUmmrP9PQVvd7evy4fy6eX-cbl4KjVGDJXKEsbZjKjGSlpTqxjXnHClCGazBlsMNSVaYYsIYVoZ0jCFmpoqY5UkluApuBjn9sG_b01MYu23ocuWYoYxqhGuGc3U9Ujp4GMMxoo-uFaGT4GgGAIWazGkKIYUxRCw2AcsPrL4cm8ho5Ybm7-vXfyZUM8hZCxjNyO2cxvz-Q8DcXf7nPecgvmo99v-D3X523pf0-Oh6g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2331813876</pqid></control><display><type>article</type><title>Ammonium addition inhibits 13C-methane incorporation into methanotroph membrane lipids in a freshwater sediment</title><source>Open Access: Oxford University Press Open Journals</source><creator>Nold, Stephen C. ; Boschker, Henricus T.S. ; Pel, Roel ; Laanbroek, Hendrikus J.</creator><creatorcontrib>Nold, Stephen C. ; Boschker, Henricus T.S. ; Pel, Roel ; Laanbroek, Hendrikus J.</creatorcontrib><description>Abstract
To investigate the effect of ammonium addition on the species composition and activity of freshwater methane oxidizing bacteria, intact sediment cores were labeled with 13CH4 and incubated under ambient and elevated ammonium concentrations. After 7 days, methanotroph activity was assessed by quantifying the isotopic composition of the carbon in membrane lipids. The 16-carbon rather than the 18-carbon methanotroph-specific biomarkers showed a clear enrichment in 13C, suggesting the importance of group I methanotrophs in these sediments. Ammonium addition resulted in a depleted isotopic signal compared to ambient controls, suggesting that high ammonium concentrations inhibit methane incorporation into cellular components. These results compare favorably with studies that showed ammonium inhibition of methane oxidation, and extend these findings by demonstrating the effect of nitrogen fertilization on methanotroph lipid synthesis.</description><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1111/j.1574-6941.1999.tb00600.x</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Ammonium ; Animal, plant and microbial ecology ; Bacteriology ; Biological and medical sciences ; Biomarkers ; Carbon ; Carbon 13 ; Composition ; Cores ; Ecology ; Fertilization ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Lipid ; Lipids ; Membranes ; Metabolism. Enzymes ; Methane ; Methanotrophic bacteria ; Microbial ecology ; Microbiology ; Oxidation ; Sediment ; Sediments ; Species composition ; Stable carbon isotope ; Various environments (extraatmospheric space, air, water)</subject><ispartof>FEMS microbiology ecology, 1999-05, Vol.29 (1), p.81-89</ispartof><rights>1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. 1999</rights><rights>1999 INIST-CNRS</rights><rights>1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3171-bf479724bdfa686fb79c949bb4372d3f30c64cb3f1447cbe4d7b1d86befba4f43</citedby><cites>FETCH-LOGICAL-c3171-bf479724bdfa686fb79c949bb4372d3f30c64cb3f1447cbe4d7b1d86befba4f43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1850077$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Nold, Stephen C.</creatorcontrib><creatorcontrib>Boschker, Henricus T.S.</creatorcontrib><creatorcontrib>Pel, Roel</creatorcontrib><creatorcontrib>Laanbroek, Hendrikus J.</creatorcontrib><title>Ammonium addition inhibits 13C-methane incorporation into methanotroph membrane lipids in a freshwater sediment</title><title>FEMS microbiology ecology</title><description>Abstract
To investigate the effect of ammonium addition on the species composition and activity of freshwater methane oxidizing bacteria, intact sediment cores were labeled with 13CH4 and incubated under ambient and elevated ammonium concentrations. After 7 days, methanotroph activity was assessed by quantifying the isotopic composition of the carbon in membrane lipids. The 16-carbon rather than the 18-carbon methanotroph-specific biomarkers showed a clear enrichment in 13C, suggesting the importance of group I methanotrophs in these sediments. Ammonium addition resulted in a depleted isotopic signal compared to ambient controls, suggesting that high ammonium concentrations inhibit methane incorporation into cellular components. These results compare favorably with studies that showed ammonium inhibition of methane oxidation, and extend these findings by demonstrating the effect of nitrogen fertilization on methanotroph lipid synthesis.</description><subject>Ammonium</subject><subject>Animal, plant and microbial ecology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Carbon</subject><subject>Carbon 13</subject><subject>Composition</subject><subject>Cores</subject><subject>Ecology</subject><subject>Fertilization</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lipid</subject><subject>Lipids</subject><subject>Membranes</subject><subject>Metabolism. Enzymes</subject><subject>Methane</subject><subject>Methanotrophic bacteria</subject><subject>Microbial ecology</subject><subject>Microbiology</subject><subject>Oxidation</subject><subject>Sediment</subject><subject>Sediments</subject><subject>Species composition</subject><subject>Stable carbon isotope</subject><subject>Various environments (extraatmospheric space, air, water)</subject><issn>0168-6496</issn><issn>1574-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqVkM1OAyEUhYnRxFp9h4m6nREKhcHERdP4l2jc6JoAAylNZxiBpvr2MplGN7qQDeHe79xzOQCcI1ihfK7WFZozUlJOUIU451VSEFIIq48DMPluHYIJRLQuKeH0GJzEuIYQzTGBE-AXbes7t20L2TQuOd8Vrls55VIsEF6WrUkr2Zlc1D70Psg9knwxtnwKvl_lR6vCAG5c75qYiUIWNpi42slkQhFN41rTpVNwZOUmmrP9PQVvd7evy4fy6eX-cbl4KjVGDJXKEsbZjKjGSlpTqxjXnHClCGazBlsMNSVaYYsIYVoZ0jCFmpoqY5UkluApuBjn9sG_b01MYu23ocuWYoYxqhGuGc3U9Ujp4GMMxoo-uFaGT4GgGAIWazGkKIYUxRCw2AcsPrL4cm8ho5Ybm7-vXfyZUM8hZCxjNyO2cxvz-Q8DcXf7nPecgvmo99v-D3X523pf0-Oh6g</recordid><startdate>199905</startdate><enddate>199905</enddate><creator>Nold, Stephen C.</creator><creator>Boschker, Henricus T.S.</creator><creator>Pel, Roel</creator><creator>Laanbroek, Hendrikus J.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>RC3</scope></search><sort><creationdate>199905</creationdate><title>Ammonium addition inhibits 13C-methane incorporation into methanotroph membrane lipids in a freshwater sediment</title><author>Nold, Stephen C. ; Boschker, Henricus T.S. ; Pel, Roel ; Laanbroek, Hendrikus J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3171-bf479724bdfa686fb79c949bb4372d3f30c64cb3f1447cbe4d7b1d86befba4f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Ammonium</topic><topic>Animal, plant and microbial ecology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Carbon</topic><topic>Carbon 13</topic><topic>Composition</topic><topic>Cores</topic><topic>Ecology</topic><topic>Fertilization</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lipid</topic><topic>Lipids</topic><topic>Membranes</topic><topic>Metabolism. Enzymes</topic><topic>Methane</topic><topic>Methanotrophic bacteria</topic><topic>Microbial ecology</topic><topic>Microbiology</topic><topic>Oxidation</topic><topic>Sediment</topic><topic>Sediments</topic><topic>Species composition</topic><topic>Stable carbon isotope</topic><topic>Various environments (extraatmospheric space, air, water)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nold, Stephen C.</creatorcontrib><creatorcontrib>Boschker, Henricus T.S.</creatorcontrib><creatorcontrib>Pel, Roel</creatorcontrib><creatorcontrib>Laanbroek, Hendrikus J.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nold, Stephen C.</au><au>Boschker, Henricus T.S.</au><au>Pel, Roel</au><au>Laanbroek, Hendrikus J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ammonium addition inhibits 13C-methane incorporation into methanotroph membrane lipids in a freshwater sediment</atitle><jtitle>FEMS microbiology ecology</jtitle><date>1999-05</date><risdate>1999</risdate><volume>29</volume><issue>1</issue><spage>81</spage><epage>89</epage><pages>81-89</pages><issn>0168-6496</issn><eissn>1574-6941</eissn><abstract>Abstract
To investigate the effect of ammonium addition on the species composition and activity of freshwater methane oxidizing bacteria, intact sediment cores were labeled with 13CH4 and incubated under ambient and elevated ammonium concentrations. After 7 days, methanotroph activity was assessed by quantifying the isotopic composition of the carbon in membrane lipids. The 16-carbon rather than the 18-carbon methanotroph-specific biomarkers showed a clear enrichment in 13C, suggesting the importance of group I methanotrophs in these sediments. Ammonium addition resulted in a depleted isotopic signal compared to ambient controls, suggesting that high ammonium concentrations inhibit methane incorporation into cellular components. These results compare favorably with studies that showed ammonium inhibition of methane oxidation, and extend these findings by demonstrating the effect of nitrogen fertilization on methanotroph lipid synthesis.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1574-6941.1999.tb00600.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-6496 |
| ispartof | FEMS microbiology ecology, 1999-05, Vol.29 (1), p.81-89 |
| issn | 0168-6496 1574-6941 |
| language | eng |
| recordid | cdi_proquest_journals_2331813876 |
| source | Open Access: Oxford University Press Open Journals |
| subjects | Ammonium Animal, plant and microbial ecology Bacteriology Biological and medical sciences Biomarkers Carbon Carbon 13 Composition Cores Ecology Fertilization Freshwater Fundamental and applied biological sciences. Psychology Lipid Lipids Membranes Metabolism. Enzymes Methane Methanotrophic bacteria Microbial ecology Microbiology Oxidation Sediment Sediments Species composition Stable carbon isotope Various environments (extraatmospheric space, air, water) |
| title | Ammonium addition inhibits 13C-methane incorporation into methanotroph membrane lipids in a freshwater sediment |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T02%3A48%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ammonium%20addition%20inhibits%2013C-methane%20incorporation%20into%20methanotroph%20membrane%20lipids%20in%20a%20freshwater%20sediment&rft.jtitle=FEMS%20microbiology%20ecology&rft.au=Nold,%20Stephen%20C.&rft.date=1999-05&rft.volume=29&rft.issue=1&rft.spage=81&rft.epage=89&rft.pages=81-89&rft.issn=0168-6496&rft.eissn=1574-6941&rft_id=info:doi/10.1111/j.1574-6941.1999.tb00600.x&rft_dat=%3Cproquest_cross%3E2331813876%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c3171-bf479724bdfa686fb79c949bb4372d3f30c64cb3f1447cbe4d7b1d86befba4f43%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2331813876&rft_id=info:pmid/&rft_oup_id=10.1111/j.1574-6941.1999.tb00600.x&rfr_iscdi=true |